Gao Fei, Chen Bo, Jiao Juan, Jia Lijia, Liu Cuimin
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Department of Clinical Laboratory, PLA Army General Hospital, Beijing, PR China.
PLoS One. 2017 Jan 19;12(1):e0170439. doi: 10.1371/journal.pone.0170439. eCollection 2017.
Vesicle-inducing protein in plastids 1 (Vipp1) is thought to play an important role both in thylakoid biogenesis and chloroplast envelope maintenance during stress. Vipp1 is conserved in photosynthetic organisms and forms a high homo-oligomer complex structure that may help sustain the membrane integrity of chloroplasts. This study cloned two novel VIPP1 genes from Triticum urartu and named them TuVipp1 and TuVipp2. Both proteins shared high identity with the homologous proteins AtVipp1 and CrVipp1. TuVipp1 and TuVipp2 were expressed in various organs of common wheat, and both genes were induced by light and various stress treatments. Purified TuVipp1 and TuVipp2 proteins showed secondary and advanced structures similar to those of the homologous proteins. Similar to AtVipp1, TuVipp1 is a chloroplast target protein. Additionally, TuVipp1 was able to rescue the phenotypes of pale leaves, lethality, and disordered chloroplast structures of AtVipp1 (-/-) mutant lines. Collectively, our data demonstrate that TuVipp1 and TuVipp2 are functional proteins in chloroplasts in wheat and may be critical for maintaining the chloroplast envelope under stress and membrane biogenesis upon photosynthesis.
质体中诱导囊泡蛋白1（Vipp1）被认为在应激期间的类囊体生物发生和叶绿体包膜维持中都发挥着重要作用。Vipp1在光合生物中保守，并形成一种高度同源寡聚体复合物结构，这可能有助于维持叶绿体的膜完整性。本研究从小麦野生二粒小麦中克隆了两个新的VIPP1基因，并将它们命名为TuVipp1和TuVipp2。这两种蛋白质与同源蛋白AtVipp1和CrVipp1具有高度同源性。TuVipp1和TuVipp2在普通小麦的各个器官中表达，并且这两个基因都受到光和各种应激处理的诱导。纯化的TuVipp1和TuVipp2蛋白显示出与同源蛋白相似的二级和高级结构。与AtVipp1相似，TuVipp1是一种叶绿体靶向蛋白。此外，TuVipp1能够挽救AtVipp1（-/-）突变株系的叶片苍白、致死性和叶绿体结构紊乱的表型。总体而言，我们的数据表明TuVipp1和TuVipp2是小麦叶绿体中的功能蛋白，并且可能对于在应激下维持叶绿体包膜以及光合作用时的膜生物发生至关重要。
