LaRocca R V, Rosenblum M, Westermark B, Israel M A
Medicine Branch, National Cancer Institute, Bethesda, MD 20892.
J Neurosci Res. 1989 Sep;24(1):97-106. doi: 10.1002/jnr.490240114.
Previously reported studies have suggested that variations in the pattern of proto-oncogene expression within a specific tumor type may denote an underlying difference in the biology and clinical behavior of those tumors. To more sensitively characterize malignant tumors of the central nervous system, we have used Northern blot hybridization analysis to determine the patterns of expression of seven proto-oncogenes in 20 cell lines established from biopsy specimens of patients with malignant glioma. The following proto-oncogenes are expressed at detectable levels in 30 micrograms of total RNA from most glioma cell lines examined: c-myc (20/20), c-mil/raf-1 (18/18), neu (19/20), c-erbB (19/20), and c-myb (17/20). In contrast, only half of the cell lines expressed detectable c-sis (10/20). In none of the cell lines tested was N-myc (0/20) mRNA detected. Morphologic analysis of these 20 cell lines revealed three different growth patterns: bipolar, epithelial, and pleomorphic-glial. Detectable levels of c-sis mRNA typically occurred with either an epithelial or pleomorphic-glial morphology. The pleomorphic-glial subgroup was also characterized by the expression of glial fibrillary acidic protein.
先前报道的研究表明，特定肿瘤类型中原癌基因表达模式的变化可能意味着这些肿瘤在生物学特性和临床行为上存在潜在差异。为了更敏感地鉴定中枢神经系统恶性肿瘤，我们使用Northern印迹杂交分析来确定从恶性神经胶质瘤患者活检标本建立的20个细胞系中7种原癌基因的表达模式。在检测的大多数神经胶质瘤细胞系的30微克总RNA中，以下原癌基因以可检测水平表达：c-myc（20/20）、c-mil/raf-1（18/18）、neu（19/20）、c-erbB（19/20）和c-myb（17/20）。相比之下，只有一半的细胞系表达可检测到的c-sis（10/20）。在所测试的细胞系中均未检测到N-myc（0/20）mRNA。对这20个细胞系的形态学分析揭示了三种不同的生长模式：双极型、上皮型和多形性胶质型。可检测水平的c-sis mRNA通常出现在上皮型或多形性胶质型形态中。多形性胶质亚组的特征还在于胶质纤维酸性蛋白的表达。
