Engelhard H H, Wolters M, Criswell P S
Department of Surgery, Northwestern University Medical School, Chicago, Illinois 60611, USA.
J Neurooncol. 1995;23(1):31-40. doi: 10.1007/BF01058457.
This study was designed to determine whether or not overexpression of the c-erbB2 protein plays a role in the etiology of human gliomas. The c-erbB2 gene codes for a 185 kDa cell membrane glycoprotein (gp185c-erbB2), which is similar to the receptor for epidermal growth factor. In initial studies, four human glioma cell lines (A-172, U118MG, U138MG and SW608) were used to develop techniques for detecting and quantifying gp185c-erbB2, using immunofluorescence microscopy, immunoblot analysis and flow cytometry. A-172 cells were found to have the highest content of gp185c-erbB2. More detailed studies utilizing A-172 cells indicated that cellular gp185c-erbB2 content changed little in response to conditions affecting cellular proliferative status, including serum deprivation, growth in low glucose medium and treatment with dimethyl sulfoxide. Ten human glioma specimens were then analyzed for cellular gp185c-erbB2 fluorescence and DNA content, using A-172 cells as a biological standard. Results indicated that gp185c-erbB2 was expressed at levels comparable to that of A-172 cells in many specimens, and at a very high level in one specimen. These data reiterate the problem of the molecular heterogeneity of human gliomas and indicate that gp185c-erbB2 may have a role in at least a subset of malignant glial tumors.
本研究旨在确定c-erbB2蛋白的过表达是否在人类胶质瘤的病因学中起作用。c-erbB2基因编码一种185 kDa的细胞膜糖蛋白（gp185c-erbB2），它与表皮生长因子受体相似。在初步研究中，使用四种人类胶质瘤细胞系（A-172、U118MG、U138MG和SW608），采用免疫荧光显微镜、免疫印迹分析和流式细胞术开发检测和定量gp185c-erbB2的技术。发现A-172细胞中gp185c-erbB2的含量最高。利用A-172细胞进行的更详细研究表明，细胞gp185c-erbB2含量对影响细胞增殖状态的条件（包括血清剥夺、在低葡萄糖培养基中生长和用二甲亚砜处理）反应变化不大。然后，以A-172细胞作为生物学标准，分析了十个人类胶质瘤标本的细胞gp185c-erbB2荧光和DNA含量。结果表明，在许多标本中，gp185c-erbB2的表达水平与A-172细胞相当，在一个标本中表达水平非常高。这些数据再次强调了人类胶质瘤分子异质性的问题，并表明gp185c-erbB2可能至少在一部分恶性胶质瘤中起作用。
