Hero Johan S, Pisa José H, Perotti Nora I, Romero Cintia M, Martínez María A
a PROIMI Planta Piloto de Procesos Industriales Microbiológicos , CONICET , Tucumán , Argentina.
b Facultad de Ciencias Exactas y Tecnología , Universidad Nacional de Tucumán , Tucumán , Argentina.
Prep Biochem Biotechnol. 2017 Jul 3;47(6):589-596. doi: 10.1080/10826068.2017.1280826. Epub 2017 Jan 20.
The behavior of three isolates retrieved from different cellulolytic consortia, Bacillus sp. AR03, Paenibacillus sp. AR247 and Achromobacter sp. AR476-2, were examined individually and as co-cultures in order to evaluate their ability to produce extracellular cellulases and xylanases. Utilizing a peptone-based medium supplemented with carboxymethyl cellulose (CMC), an increase estimation of 1.30 and 1.50 times was obtained by the co-culture containing the strains AR03 and AR247, with respect to enzyme titles registered by their individual cultivation. On the contrary, the extracellular enzymatic production decreased during the co-cultivation of strain AR03 with the non-cellulolytic Achromobacter sp. AR476-2. The synergistic behavior observed through the combined cultivation of the strains AR03 and AR247 might be a consequence of the consumption by Paenibacillus sp. AR247 of the products of the CMC hydrolysis (i.e., cellobiose and/or cello-oligosaccharides), which were mostly generated by the cellulase producer Bacillus sp. AR03. The effect observed could be driven by the requirement to fulfill the nutritional supply from both strains on the substrate evaluated. These results would contribute to a better description of the degradation of the cellulose fraction of the plant cell walls in nature, expected to an efficient utilization of renewable sources.
对从不同纤维素分解菌群中分离出的三株菌，即芽孢杆菌属AR03、类芽孢杆菌属AR247和无色杆菌属AR476-2，分别进行了单独培养和共培养，以评估它们产生胞外纤维素酶和木聚糖酶的能力。在添加了羧甲基纤维素（CMC）的蛋白胨培养基中，含有AR03和AR247菌株的共培养物所产生的酶量，相对于它们单独培养时记录的酶量，估计增加了1.30倍和1.50倍。相反，在AR03菌株与非纤维素分解菌无色杆菌属AR476-2共培养期间，胞外酶产量下降。通过AR03和AR247菌株的联合培养观察到的协同行为，可能是由于类芽孢杆菌属AR247消耗了CMC水解产物（即纤维二糖和/或纤维寡糖），而这些产物大多是由纤维素酶产生菌芽孢杆菌属AR03产生的。观察到的这种效应可能是由于两株菌对所评估底物的营养供应需求所驱动的。这些结果将有助于更好地描述自然界中植物细胞壁纤维素部分的降解情况，有望实现对可再生资源的高效利用。
