着丝粒稳定性：复制关联

Forsburg Susan L, Shen Kuo-Fang

Program in Molecular & Computational Biology, University of Southern California, Los Angeles, CA 90089-2910, USA.

Genes (Basel). 2017 Jan 18;8(1):37. doi: 10.3390/genes8010037.

The fission yeast centromere, which is similar to metazoan centromeres, contains highly repetitive pericentromere sequences that are assembled into heterochromatin. This is required for the recruitment of cohesin and proper chromosome segregation. Surprisingly, the pericentromere replicates early in the S phase. Loss of heterochromatin causes this domain to become very sensitive to replication fork defects, leading to gross chromosome rearrangements. This review examines the interplay between components of DNA replication, heterochromatin assembly, and cohesin dynamics that ensures maintenance of genome stability and proper chromosome segregation.

裂殖酵母着丝粒与后生动物着丝粒相似，包含高度重复的着丝粒周围序列，这些序列组装成异染色质。这是募集黏连蛋白和正确进行染色体分离所必需的。令人惊讶的是，着丝粒周围区域在S期早期复制。异染色质的缺失导致该区域对复制叉缺陷变得非常敏感，从而导致大规模的染色体重排。本综述探讨了DNA复制、异染色质组装和黏连蛋白动力学之间的相互作用，这些相互作用确保了基因组稳定性的维持和染色体的正确分离。