Goryacheva O A, Beloglazova N V, Vostrikova A M, Pozharov M V, Sobolev A M, Goryacheva I Yu
Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia.
Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia; Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
Talanta. 2017 Mar 1;164:377-385. doi: 10.1016/j.talanta.2016.11.054. Epub 2016 Nov 30.
Förster resonance energy transfer (FRET) between lanthanide ion complexes (L) acting as donors and luminescent semiconductor quantum dots (QD) acting as acceptors is discussed in the terms of advantages and disadvantages for its application in immunoassay. L-QD-FRET is potentially a powerful tool that can be used to detect and confirm formation of immunocomplexes, but until now it had very limited practical analytical application. Therefore, the main aim of this review is to analyze all possibilities, advantages, and disadvantages of L-QD-FRET in immunoassay applications. Considering L and QD respectively applied as donor and acceptor, the most advantageous properties for analytical purposes are large decay time of L complexes and the high absorption of QD. L complexes' extremely long decay times make it possible to directly detect FRET through enhancement of QDs decay time as a result of energy transfer. Very high QD absorption predetermines extremely large Förster radii (ca. 10nm), which means that FRET can be utilized for proteins and protein complexes, such as antigen-antibody systems.
本文讨论了以镧系离子配合物（L）作为供体和发光半导体量子点（QD）作为受体之间的荧光共振能量转移（FRET）在免疫分析应用中的优缺点。L-QD-FRET可能是一种强大的工具，可用于检测和确认免疫复合物的形成，但到目前为止，其实际分析应用非常有限。因此，本综述的主要目的是分析L-QD-FRET在免疫分析应用中的所有可能性、优点和缺点。分别考虑将L和QD用作供体和受体，用于分析目的的最有利特性是L配合物的长衰减时间和QD的高吸收性。L配合物极长的衰减时间使得通过能量转移增强QD衰减时间来直接检测FRET成为可能。非常高的QD吸收性决定了极大的福斯特半径（约10nm），这意味着FRET可用于蛋白质和蛋白质复合物，如抗原-抗体系统。
