Panferov Vasily G, Safenkova Irina V, Zherdev Anatoly V, Dzantiev Boris B
A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
Talanta. 2017 Mar 1;164:69-76. doi: 10.1016/j.talanta.2016.11.025. Epub 2016 Nov 13.
A barcode lateral flow immunoassay (LFIA) based on magnetic nanoparticles with a controllable cut-off level was developed for the first time. The regulation of the cut-off levels was based on the changes in the position and concentration of three antibodies (two monoclonal and polyclonal) with different affinities (K=4.22×10, 9.67×10, 1.05×10 M), respectively. To obtain specific conjugates, monoclonal antibodies were covalently immobilized on the magnetic nanoparticles' surface. Potato virus X causing a reduction in the potato yield was used as a model polyvalent antigen. To detect potato virus X in the leaf extracts, a barcode LFIA with cut-off levels of 3, 30, and 150ng/mL was used for the analysis. The application of magnetic concentration leads to a six-fold reduction in the first cut-off level (0.5ng/mL) in comparison with magnetic LFIA without the concentration stage.
首次开发了一种基于磁性纳米颗粒的具有可控截断水平的条形码侧向流动免疫分析（LFIA）。截断水平的调节分别基于三种具有不同亲和力（K = 4.22×10、9.67×10、1.05×10 M）的抗体（两种单克隆抗体和多克隆抗体）的位置和浓度变化。为了获得特异性缀合物，将单克隆抗体共价固定在磁性纳米颗粒表面。导致马铃薯产量下降的马铃薯X病毒用作模型多价抗原。为了检测叶片提取物中的马铃薯X病毒，使用截断水平为3、30和150ng/mL的条形码LFIA进行分析。与没有浓缩阶段的磁性LFIA相比，磁性浓缩的应用使第一个截断水平（0.5ng/mL）降低了六倍。
