Munir Shehla, Ahmed Sarfraz, Ibrahim Muhammad, Khalid Muhammad, Ojha Suvash Chandra
Department of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.
Department of Basic Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Front Bioeng Biotechnol. 2020 Sep 8;8:883. doi: 10.3389/fbioe.2020.00883. eCollection 2020.
Great scientific research with improved potential in probing biological locales has remained a giant stride. The use of bio-barcodes with the potential use of nanotechnology is a hallmark being developed among recent advanced techniques. Biobarcoding is a novel method used for screening biomolecules to identify and divulge ragbag biodiversity. It establishes successful barcoding projects in the field of nanomedical technology for massively testing disease diagnosis and treatment. Biobarcoding and nanotechnology are recently developed technologies that provide unique opportunities and challenges for multiplex detection such as DNAs, proteins and nucleic acids of animals, plants, viruses, and various other species. These technologies also clump drug delivery, gene delivery, and DNA sequencing. Bio-barcode amplification assay (BCA) is used at large for the detection and identification of proteins and DNAs. DNA barcoding combined with nanotechnology has been proven highly sensitive rendering fast uniplex and multiplex detection of pathogens in food, blood, and other specimens. This review takes a panoramic view of current advances in nano bio-barcodes which have been summarized to explore additional applications such as detection of cytokines, neurotransmitters, cancer markers, prostate-specific antigens, and allergens. In the future, it will also be possible to detect some fungi, algae, protozoa, and other pollutants in food, agriculture, and clinical samples. Using these technologies, specific and efficient sensors would possibly be developed that can perform swift detections of antigens, allergens, and other specimens.
在探索生物区域方面具有更高潜力的伟大科学研究一直是巨大的进步。使用具有纳米技术潜在用途的生物条形码是最近先进技术中正在发展的一个标志。生物条形码是一种用于筛选生物分子以识别和揭示杂乱无章的生物多样性的新方法。它在纳米医学技术领域建立了成功的条形码项目，用于大规模测试疾病诊断和治疗。生物条形码和纳米技术是最近开发的技术，为诸如动物、植物、病毒和各种其他物种的DNA、蛋白质和核酸等多重检测提供了独特的机遇和挑战。这些技术还涉及药物递送、基因递送和DNA测序。生物条形码扩增测定法（BCA）广泛用于蛋白质和DNA的检测与鉴定。DNA条形码与纳米技术相结合已被证明具有高度敏感性，能够快速进行单重和多重检测食品、血液和其他样本中的病原体。本综述全面审视了纳米生物条形码的当前进展，这些进展已被总结以探索其他应用，如细胞因子、神经递质、癌症标志物、前列腺特异性抗原和过敏原的检测。未来，还将有可能检测食品、农业和临床样本中的一些真菌、藻类、原生动物和其他污染物。利用这些技术，可能会开发出能够快速检测抗原、过敏原和其他样本的特异性高效传感器。
