[建立外阴慢性单纯性苔藓的SD大鼠模型及蛋白酶激活受体2表达的检测]
[Establishment of a SD rat model of vulvar lichen simplex chronicus and detection of the expression of protease activated receptor 2].
作者信息
Yang Huan, Fu Zhen-Hua, Tang Hua-Jun, Li Cheng-Zhi
机构信息
State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Collaborative Innovation Center for Minimally-invasive and Noninvasive Medicine; Chongqing Medical University, Chongqing 40016, China.E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Jan 20;37(1):30-35. doi: 10.3969/j.issn.1673-4254.2017.01.06.
OBJECTIVE
To establish a SD rat model of vulvar lichen simplex chronicus (LSC) and investigate the expression of protease activated receptor 2 (PAR2) in the genital skin.
METHODS
Seventy female SD rats were randomly divided into group A (blank control group, n=10), group B (with application of acetone solution 3 times per week for 10 weeks, n=10), group C (with chronic mechanical irritation 3 times per week for 10 weeks, n=10), and group D (with topical treatment with 0.5= 7,12-Dimethylbenzanthracene [DMBA] in acetone solution and chronic mechanical irritation 3 times per week for 10 weeks, n=40). The changes of the genital skin changes were observed regularly and the expression of PAR2 in groups A and D was detected with immunohistochemistry, Western blotting and qRT-PCR.
RESULTS
In group D, LSC occurred in 23 rats (57.5=) at 8 weeks and in 38 rats (95=) at 10 weeks; 8 rats (20=) showed papilloma at 12 weeks. Acetone treatment or chronic mechanical irritation did not cause LSC in the rats. Immunohistochemistry, Western blotting and qRT-PCR showed significantly increased expressions of PAR2 in group D at both the protein and mRNA levels as compared with those in group A (P<0.05).
CONCLUSION
0.5= DMBA in acetone solution along with chronic mechanical irritation can induce LSC in female SD rats, and PAR2 is closely related with the occurrence and progression of LSC.
目的
建立雌性SD大鼠外阴慢性单纯性苔藓（LSC）模型，探讨蛋白酶激活受体2（PAR2）在生殖器皮肤中的表达。
方法
将70只雌性SD大鼠随机分为A组（空白对照组，n = 10）、B组（每周外用丙酮溶液3次，共10周，n = 10）、C组（每周进行慢性机械刺激3次，共10周，n = 10）和D组（外用0.5% 7,12 - 二甲基苯并蒽[DMBA]丙酮溶液并每周进行慢性机械刺激3次，共10周，n = 40）。定期观察生殖器皮肤变化，采用免疫组织化学、蛋白质印迹法及实时荧光定量PCR检测A组和D组PAR2的表达。
结果
D组在8周时23只大鼠（57.5%）发生LSC，10周时38只大鼠（95%）发生LSC；12周时8只大鼠（20%）出现乳头瘤。丙酮处理或慢性机械刺激未导致大鼠发生LSC。免疫组织化学、蛋白质印迹法及实时荧光定量PCR结果显示，与A组相比，D组PAR2在蛋白和mRNA水平的表达均显著增加（P < 0.05）。
结论
0.5% DMBA丙酮溶液联合慢性机械刺激可诱导雌性SD大鼠发生LSC，PAR2与LSC的发生发展密切相关。
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