Liu Yao, Fan Yijin, Li Chengzhi
State Key Laboratory of Ultrasound Medical Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China.
Chongqing Key Laboratory of Biomedical Engineering, Chongqing 400016, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Dec 30;39(12):1487-1493. doi: 10.12122/j.issn.1673-4254.2019.12.14.
To explore the changes of collagen fibrosis in the vulva skin of SD rats with lichen simplex chronicus (LSC) after focused ultrasound therapy and explore the mechanism by which focused ultrasound reduces the recurrence of vulvar LSC.
Fifty female SD rat models of vulvar LSC were established and randomly divided into the treatment group and the control group (=25) for treatment with focused ultrasound and sham treatment, respectively. Before and after the treatment, vulvar skin tissues were sampled to observe the pathological changes with HE staining and assess the density of collagen fibers using Masson staining. The ultrastructure of the collagen fibers in the superficial dermis was observed using transmission electron microscopy. The expressions of notch1 and c-fos in the vulvar tissue were detected by immunohistochemistry and Western blotting.
After 4 weeks of focused ultrasound therapy, 16% (4/25) of the rats in the treatment group showed lesion progression to LSIL, 4% (1/25) still had LSC, and 80% (20/25) showed normal vulvar skin. In the control group, progression to LSIL occurred in 19 (76%) rats, 3 (12%) rats still showed LSC, and only 3 (12%) had normal vulvar skin. The difference in the cure rate differed significantly between the two groups ( < 0.05). The density of collagen fibers in the superficial dermis and the expressions of notch1 and c-fos in the vulvar skin were significantly lower in the treatment group than in the control group ( < 0.05).
Focused ultrasound therapy can inhibit superficial collagen fibrosis of the dermis by lowering the expressions of notch1 and c-fos in the vulvar skin to reduce the recurrence of vulvar LSC in rats.
探讨聚焦超声治疗后慢性单纯性苔藓（LSC）模型SD大鼠外阴皮肤胶原纤维化的变化，探讨聚焦超声降低外阴LSC复发率的机制。
建立50只雌性外阴LSC的SD大鼠模型，随机分为治疗组和对照组（每组25只），分别接受聚焦超声治疗和假治疗。治疗前后取外阴皮肤组织，行HE染色观察病理变化，采用Masson染色评估胶原纤维密度。用透射电子显微镜观察真皮浅层胶原纤维的超微结构。通过免疫组织化学和蛋白质印迹法检测外阴组织中notch1和c-fos的表达。
聚焦超声治疗4周后，治疗组16%（4/25）的大鼠病变进展为低度鳞状上皮内病变（LSIL），4%（1/25）仍为LSC，80%（20/25）外阴皮肤正常。对照组中，19只（76%）大鼠进展为LSIL，3只（12%）大鼠仍为LSC，仅3只（12%）外阴皮肤正常。两组治愈率差异有统计学意义（P<0.05）。治疗组真皮浅层胶原纤维密度及外阴皮肤中notch1和c-fos的表达均显著低于对照组（P<0.05）。
聚焦超声治疗可通过降低外阴皮肤中notch1和c-fos的表达，抑制真皮浅层胶原纤维化，从而降低大鼠外阴LSC的复发率。
