Madeiro Sara A L, Borges Nathalie H P B, Souto Augusto L, de Figueiredo Pedro T R, Siqueira-Junior José P, Tavares Josean F
Centro de Biotecnologia, Universidade Federal da Paraíba, João Pessoa, Brazil.
Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Brazil.
Microb Pathog. 2017 Mar;104:151-154. doi: 10.1016/j.micpath.2017.01.028. Epub 2017 Jan 19.
The first occurrences and dissemination of resistant microorganisms led to the inefficacy of many antibiotics, available in the market nowadays, therefore, the search for new substances with antimicrobial activity from natural sources has gained a great importance. The purpose of this work is to evaluate the antibacterial activity and modulation of drug resistance in Staphylococcus aureus by coumarins such as bergapten, xantotoxin, isopimpinellin and imperatorin obtained from two Rutaceae species (Metrodorea mollis and Pilocarpus spicatus). The antimicrobial activity was assessed based on the minimum inhibitory concentration (MIC), using the microdilution method. The MIC was >256 g/mL for all coumarins tested. Regarding the modulation of drug resistance assay, the isopimpinellin reducted the MIC of erytromicin by 4 times, whereas imperatorin exhibited the best result, reducing the MIC of tetracycline (2 times), erytomicin (4 times) and norfloxacin (4 times). By reducing the MIC of ethidium bromide, the imperatorin is consider in fact, as a putative efflux pump inhibitor of bacteria.
耐药微生物的首次出现和传播导致了当今市场上许多抗生素失效，因此，从天然来源寻找具有抗菌活性的新物质变得极为重要。本研究的目的是评估从两种芸香科植物（软肉美苦木和穗花毛果芸香）中获得的香豆素（如补骨脂素、花椒毒素、异茴芹素和欧前胡素）对金黄色葡萄球菌的抗菌活性及耐药性调节作用。采用微量稀释法，基于最低抑菌浓度（MIC）评估抗菌活性。所有测试香豆素的MIC均>256μg/mL。关于耐药性调节试验，异茴芹素使红霉素的MIC降低了4倍，而欧前胡素表现最佳，使四环素的MIC降低了2倍，红霉素的MIC降低了4倍，诺氟沙星的MIC降低了4倍。通过降低溴化乙锭的MIC，欧前胡素实际上被认为是一种假定的细菌外排泵抑制剂。
