Laboratório de Microbiologia e Biologia Molecular- LMBM, Universidade Regional do Cariri- URCA, Crato, Ceará, Brazil.
Laboratório de Microbiologia e Biologia Molecular- LMBM, Universidade Regional do Cariri- URCA, Crato, Ceará, Brazil.
Microb Pathog. 2017 Dec;113:265-268. doi: 10.1016/j.micpath.2017.10.052. Epub 2017 Oct 28.
The treatment of infections caused by microorganisms that are resistant to antibiotics represent one of the main challenges of medicine today, especially due to the inefficacy of long-term drug therapy. In the search for new alternatives to treat these infections, many researchers have been looking for new substances derived from natural products to replace, or be used in combination with conventional antibiotics. Vanillin is a phenolic compound whose antimicrobial activity has been used in the elimination of pathogens present in fruits and vegetables. However, its antibacterial and modulating properties remain to be characterized. Therefore, this work aimed to evaluate the antibacterial activity and analyze the modulator activity of vanillin in association with conventional antibiotics. The antimicrobial activity of vanillin was evaluated using the microdilution method to determine the Minimum Inhibitory Concentration (MIC) Standard strains of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and multi-resistant strains of Escherichia coli 06, Staphylococcus aureus 10, Pseudomonas aeruginosa 24 were used in this study. The antibiotic modulating effect was analyzed by combining vanillin with Norfloxacin, Imipenem, Gentamicin, Erythromycin and Tetracycline against the following multiresistant bacteria strains: Escherichia coli 06, Staphylococcus aureus 10 and Pseudomonas aeruginosa 24. Data were analyzed using the ANOVA test of two tracks followed by the post hoc Bonferroni test. Vanillin presented CIMs ≥1024μg/mL against all tested strains demonstrating that it did not present significant antibacterial activity. However, modulated the activity of gentamicin and imipenem against S. aureus and E. coli, causing a synergistic effect, but did not affect the activity of norfloxacin, tetracycline and erythromycin against these same microorganisms. A synergistic effect was also obtained from the association of vanillin with norfloxacin against P. aeruginosa. On the other hand, against this strain the association of vanillin with tetracycline and erythromycin caused antagonism, although the activity of gentamicin and imipenem was not affected. In conclusion, vanillin selectively modulated the activity of antibiotics against multiresistant bacteria and as such, might be useful in the development of new therapies against resistant microorganism.
抗生素耐药微生物引起的感染的治疗是当今医学面临的主要挑战之一，尤其是由于长期药物治疗无效。为了寻找治疗这些感染的新替代方法，许多研究人员一直在寻找源自天然产物的新物质来替代或与传统抗生素联合使用。香草醛是一种酚类化合物，其抗菌活性已被用于消除水果和蔬菜中的病原体。然而，其抗菌和调节特性仍有待表征。因此，本工作旨在评估香草醛的抗菌活性，并分析其与常规抗生素联合使用的调节活性。使用微量稀释法评估香草醛的抗菌活性，以确定最小抑菌浓度（MIC）。本研究使用标准大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和多耐药大肠杆菌 06、金黄色葡萄球菌 10、铜绿假单胞菌 24 菌株进行了该实验。通过将香草醛与诺氟沙星、亚胺培南、庆大霉素、红霉素和四环素联合使用，分析了对以下多耐药细菌菌株的抗生素调节作用：大肠杆菌 06、金黄色葡萄球菌 10 和铜绿假单胞菌 24。使用双因素方差分析（ANOVA）和事后 Bonferroni 检验对数据进行了分析。香草醛对所有测试菌株的 CIMs 值均≥1024μg/ml，表明其没有显著的抗菌活性。然而，它调节了庆大霉素和亚胺培南对金黄色葡萄球菌和大肠杆菌的活性，产生协同作用，但对诺氟沙星、四环素和红霉素对这些相同微生物的活性没有影响。香草醛与诺氟沙星联合使用对铜绿假单胞菌也产生了协同作用。另一方面，对于这种菌株，香草醛与四环素和红霉素的联合使用引起了拮抗作用，尽管庆大霉素和亚胺培南的活性没有受到影响。综上所述，香草醛选择性地调节了抗生素对多耐药菌的活性，因此可能对开发针对耐药微生物的新疗法有用。
