Nagai S
Kekkaku. 1989 Sep;64(9):585-91.
More than 10 mycobacterial proteins (MPB or MPT) have been isolated from the nutrient fluids after cultivation of Mycobacterium bovis BCG (Japanese substrain) or M. tuberculosis H37Rv on Sauton medium. The genes for 4 antigens, MPB70, MPB64, alpha-antigen (MPB59), and MPB57, were cloned so far and the complete nucleotide and amino acid sequences were determined. MPB70 is a quite unique protein in 3 points; a highly species-specific antigen for M. bovis, a large amount of secretion more than 10% of the total protein amount in the culture fluid when the cells are actively growing, and a composition with hydrophobic amino acids in a high rate. MPB64 was also a unique antigen specific to M. bovis and M. tuberculosis with a strong reactivity in delayed-type hypersensitivity. MPB59, corresponds to alpha-antigen, was a protein of a group of several similar configurations and was commonly found in the mycobacterial species. These 3 antigens are synthesized in the cells binding with each signal peptide which is characteristic of secreted proteins. A heat stable protein, MPB57, a dominant component of PPD, corresponded to BCG-a or 10kD protein and is supposed to be one of the heat shock proteins.
在苏通培养基上培养牛分枝杆菌卡介苗（日本亚株）或结核分枝杆菌H37Rv后，已从营养液中分离出10多种分枝杆菌蛋白（MPB或MPT）。到目前为止，已克隆了4种抗原（MPB70、MPB64、α抗原（MPB59）和MPB57）的基因，并确定了其完整的核苷酸和氨基酸序列。MPB70在三个方面是一种相当独特的蛋白质：它是牛分枝杆菌的一种高度种特异性抗原；当细胞活跃生长时，其分泌量超过培养液中总蛋白量的10%；并且其疏水氨基酸的比例很高。MPB64也是牛分枝杆菌和结核分枝杆菌特有的一种独特抗原，在迟发型超敏反应中具有很强的反应性。MPB59对应于α抗原，是一组具有几种相似构型的蛋白质之一，常见于分枝杆菌属。这三种抗原在细胞内与分泌蛋白特有的信号肽结合进行合成。一种热稳定蛋白MPB57是PPD的主要成分，对应于卡介苗a或10kD蛋白，被认为是热休克蛋白之一。
