Chase M W, Kawata H
Dev Biol Stand. 1975;29:308-30.
Guinea-pig antisera containing IgG1 antibodies have been prepared either with killed mycobacterial in paraffin oil or living BCG or intradermal injections of fractions of unheated culture filtrate. Sera, found to contain antibodies specific for different mycobacterial antigens are used in passive cutaneous anaphylaxis (PCA) in 12 times the dilution-to-extinction titer. Animals prepared with sera respond in all sites when unheated culture filtrate, strain H37Rv, is injected intravenously (i.v.) at 17-20 hours. Injection of diagnostic tuberculins i.v. into parallel recipients, in selected amounts, reveals, through reacting sites, the presence of the corresponding antigens and some information on relative amounts. Tuberculins of PPD type are precipitated from heated culture filtrate by ammonium sulfate or trichloroacetic acid (TCA) or benzoic acid. Old Tuberculins are made classically by long heating and evaporation. In all products heat-labile antigens are absent or present in small amount; yet, PPD-S was found to possess five antigens. In all PPD products, there was one dominant antigen, absent from Old Tuberculins. The dominant antigen of OT's was excluded from the TCA-PPD's but was present in most ammonium sulfate-type PPD's. It can be concluded that diagnostic testing made with OT detects a particular delayed-type sensitivity that is different in specificity from that which reacts to TCA-PPD's. Many but not all of the ammonium sulfate PPD's test for both specificities. Several different delayed-type sensitivities are acquired during infection. Supported by Grant AI 01258 of the National Institute of Health.
已通过以下方法制备了含有IgG1抗体的豚鼠抗血清：用石蜡油中的灭活分枝杆菌、活卡介苗或皮内注射未加热培养滤液的组分。发现含有针对不同分枝杆菌抗原的抗体的血清，以稀释至效价消失滴度的12倍稀释度用于被动皮肤过敏反应（PCA）。用这些血清处理过的动物，在17 - 20小时静脉注射（i.v.）未加热培养滤液H37Rv株时，所有部位都会出现反应。向平行的受体静脉注射选定剂量的诊断性结核菌素，通过反应部位可揭示相应抗原的存在以及有关相对含量的一些信息。PPD型结核菌素可通过硫酸铵、三氯乙酸（TCA）或苯甲酸从加热的培养滤液中沉淀出来。旧结核菌素传统上是通过长时间加热和蒸发制成的。在所有产品中，不耐热抗原不存在或含量很少；然而，发现PPD - S含有五种抗原。在所有PPD产品中，有一种主要抗原，旧结核菌素中不存在。旧结核菌素的主要抗原被排除在TCA - PPD中，但存在于大多数硫酸铵型PPD中。可以得出结论，用旧结核菌素进行的诊断检测可检测到一种特定的迟发型敏感性，其特异性与对TCA - PPD反应的特异性不同。许多但并非所有硫酸铵PPD都能检测这两种特异性。感染期间会获得几种不同的迟发型敏感性。由美国国立卫生研究院资助项目AI 01258支持。
