Buck A, Aichler M, Huber K, Walch A
Research Unit Analytical Pathology, Helmholtz Zentrum München, Neuherberg, Germany.
Research Unit Analytical Pathology, Helmholtz Zentrum München, Neuherberg, Germany.
Adv Cancer Res. 2017;134:117-132. doi: 10.1016/bs.acr.2016.11.004. Epub 2016 Dec 20.
Metabolomics is a rapidly evolving and a promising research field with the expectation to improve diagnosis, therapeutic treatment prediction, and prognosis of particular diseases. Among all techniques used to assess the metabolome in biological systems, mass spectrometry imaging is the method of choice to qualitatively and quantitatively analyze metabolite distribution in tissues with a high spatial resolution, thus providing molecular data in relation to cancer histopathology. The technique is ideally suited to study tissues molecular content and is able to provide molecular biomarkers or specific mass signatures which can be used in classification or the prognostic evaluation of tumors. Recently, it was shown that FFPE tissue samples are also suitable for metabolic analyses. This progress in methodology allows access to a highly valuable resource of tissues believed to widen and strengthen metabolic discovery-driven studies.
代谢组学是一个快速发展且前景广阔的研究领域，有望改善特定疾病的诊断、治疗预测和预后。在所有用于评估生物系统中代谢组的技术中，质谱成像技术是定性和定量分析组织中代谢物分布的首选方法，具有高空间分辨率，从而提供与癌症组织病理学相关的分子数据。该技术非常适合研究组织的分子含量，能够提供可用于肿瘤分类或预后评估的分子生物标志物或特定质量特征。最近的研究表明，福尔马林固定石蜡包埋（FFPE）组织样本也适用于代谢分析。这种方法学上的进展使得能够获取被认为可拓宽和加强代谢发现驱动研究的极具价值的组织资源。
