Zhou Haisheng, Zhang Meihong, Tian Xue, Shao Hongxia, Qian Kun, Ye Jianqiang, Qin Aijian
Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China; Jiangsu Key Lab of Zoonosis, Yangzhou 225009, PR China.
Vet Microbiol. 2017 Feb;199:120-127. doi: 10.1016/j.vetmic.2016.12.038. Epub 2017 Jan 3.
The emergence of new infectious bronchitis virus (IBV) variants is often disastrous in the poultry industry. In this study, an IBV, CK/CH/2010/JT-1, was isolated from an H120- and 4/91-IBV-vaccinated flock in China. Antisera against vaccine strains H120 and 4/91 could not provide effective protection against CK/CH/2010/JT-1 in virus neutralization assays. CK/CH/2010/JT-1 could cause 43.75% mortality with respiratory and severe renal lesions in inoculated chickens. Phylogenetic analysis of the S1 gene showed that CK/CH/2010/JT-1 and 31 other isolates could be grouped as a new genotypic cluster. Recombination analysis revealed that three recombination events could be found in the genome of CK/CH/2010/JT-1 at positions 24709-365, 17160-19811 and 21136-21770. Whole-genome sequence analysis showed that CK/CH/2010/JT-1 originated from multiple template switches among QX-like, CK/CH/LSC/99I-, tl/CH/LDT3/03- and 4/91-type IBVs. All of these data demonstrated that CK/CH/2010/JT-1 is a new recombinant genotype IBV with high virulence. Our findings suggest that the surveillance of new genotype strains of IBV is very important for developing more effective anti-IBV strategies.
新型传染性支气管炎病毒（IBV）变种的出现在家禽业中往往是灾难性的。在本研究中，一株IBV，CK/CH/2010/JT - 1，从中国一个接种过H120和4/91 - IBV疫苗的鸡群中分离得到。在病毒中和试验中，针对疫苗株H120和4/91的抗血清不能为抵抗CK/CH/2010/JT - 1提供有效保护。CK/CH/2010/JT - 1可导致接种鸡43.75%的死亡率，并伴有呼吸道和严重的肾脏病变。S1基因的系统发育分析表明，CK/CH/2010/JT - 1和其他31株分离株可归为一个新的基因型簇。重组分析显示，在CK/CH/2010/JT - 1基因组的24709 - 365、17160 - 19811和21136 - 21770位置可发现三个重组事件。全基因组序列分析表明，CK/CH/2010/JT - 1起源于QX样、CK/CH/LSC/99I -、tl/CH/LDT3/03 -和4/91型IBV之间的多个模板转换。所有这些数据表明，CK/CH/2010/JT - 1是一种具有高毒力的新型重组基因型IBV。我们的研究结果表明，对IBV新基因型菌株的监测对于制定更有效的抗IBV策略非常重要。
