肽酶联免疫吸附测定 (pELISA) 作为评估 IBV 疫苗免疫反应的中和试验的一种可能替代方法。
Peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine.
机构信息
Key Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China.
Jiangsu Key Lab of Zoonosis, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China.
出版信息
BMC Vet Res. 2021 Jan 25;17(1):51. doi: 10.1186/s12917-021-02757-5.
BACKGROUND
Infectious bronchitis virus (IBV), a coronavirus, is one of the most important poultry pathogens worldwide due to its multiple serotypes and poor cross-protection. Vaccination plays a vital role in controlling the disease. The efficacy of vaccination in chicken flocks can be evaluated by detecting neutralizing antibodies with the neutralization test. However there are no simple and rapid methods for detecting the neutralizing antibodies.
RESULTS
In this study, a peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine was developed. The pELISA could indirect evaluate neutralizing antibody titers against different types of IBV in all tested sera. The titers measured with the pELISA had a coefficient of 0.83 for neutralizing antibody titers.
CONCLUSIONS
The pELISA could detect antibodies against different types of IBV in all tested sera. The pELISA has the potential to evaluate samples for IBV-specific neutralizing antibodies and surveillance the infection of IBV.
背景
传染性支气管炎病毒（IBV）是一种冠状病毒，由于其多种血清型和交叉保护作用不佳，是世界范围内最重要的家禽病原体之一。疫苗接种在控制疾病方面起着至关重要的作用。通过中和试验检测中和抗体，可以评估鸡群的疫苗接种效果。然而，目前还没有简单、快速的方法来检测中和抗体。
结果
本研究中，建立了一种肽酶联免疫吸附试验（pELISA），作为中和试验的替代方法，用于评估 IBV 疫苗的免疫反应。pELISA 可以间接评估针对不同类型 IBV 的中和抗体滴度。用 pELISA 测量的滴度与中和抗体滴度的相关系数为 0.83。
结论
pELISA 可以检测所有检测血清中针对不同类型 IBV 的抗体。pELISA 有可能评估针对 IBV 特异性中和抗体的样本，并监测 IBV 的感染。
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