Woodworth Mollie B, Girskis Kelly M, Walsh Christopher A
Division of Genetics and Genomics, Manton Center for Orphan Disease, and Howard Hughes Medical Institute, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
Departments of Neurology and Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Nat Rev Genet. 2017 Apr;18(4):230-244. doi: 10.1038/nrg.2016.159. Epub 2017 Jan 23.
Resolving lineage relationships between cells in an organism is a fundamental interest of developmental biology. Furthermore, investigating lineage can drive understanding of pathological states, including cancer, as well as understanding of developmental pathways that are amenable to manipulation by directed differentiation. Although lineage tracking through the injection of retroviral libraries has long been the state of the art, a recent explosion of methodological advances in exogenous labelling and single-cell sequencing have enabled lineage tracking at larger scales, in more detail, and in a wider range of species than was previously considered possible. In this Review, we discuss these techniques for cell lineage tracking, with attention both to those that trace lineage forwards from experimental labelling, and those that trace backwards across the life history of an organism.
解析生物体中细胞间的谱系关系是发育生物学的一项基本研究内容。此外，研究谱系有助于深入了解包括癌症在内的病理状态，以及有助于理解可通过定向分化进行调控的发育途径。尽管通过注射逆转录病毒文库进行谱系追踪长期以来一直是最先进的技术，但最近在外源标记和单细胞测序方面的一系列方法学进展，使得谱系追踪能够在比以往认为可能的更大规模、更详细以及更广泛的物种范围内进行。在本综述中，我们将讨论这些用于细胞谱系追踪的技术，既关注那些从实验标记向前追踪谱系的技术，也关注那些在生物体的生命历程中向后追溯谱系的技术。
