Gao Liang-Liang, Guo Tao, Xu Xu-Dong, Yang Jun-Shan
a Food and Drug College , Anhui Science and Technology University , Fengyang , China.
c Institute of Medicinal Plant Development , Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
Nat Prod Res. 2017 Jul;31(13):1529-1535. doi: 10.1080/14786419.2017.1280491. Epub 2017 Jan 23.
Rhubarb contains biologically active compounds such as anthraquinones, anthrones, stilbenes and tannins. A rapid and efficient UPLC/Q-TOF-MS/MS method was developed and applied towards identifying the constituents of Rheum tanguticum Maxim. ex Balf. for the first time. Chemical constituents were separated and investigated by UPLC/Q-TOF-MS/MS in the negative ion mode. The ESI-MS fragmentation pathways of four types of compounds were interpreted, providing a very useful guidance for the characterisation of different types of compounds. Based on the exact mass information, fragmentation characteristic and LC retention time of 7 reference standards, 30 constituents were tentatively identified from the methanol extract of R. tanguticum. Among them, seven compounds were described for the first time from R. tanguticum and two from the genus Rheum were described for the first time. The analytical tool used here is valuable for the rapid separation and identification of multiple and minor constituents in methanol extracts of R. tanguticum.
大黄含有蒽醌、蒽酮、芪类和单宁等生物活性化合物。首次开发并应用了一种快速高效的超高效液相色谱/四极杆飞行时间串联质谱（UPLC/Q-TOF-MS/MS）方法来鉴定唐古特大黄的成分。通过UPLC/Q-TOF-MS/MS在负离子模式下对化学成分进行分离和研究。解释了四类化合物的电喷雾电离质谱（ESI-MS）裂解途径，为不同类型化合物的表征提供了非常有用的指导。基于7种参考标准品的精确质量信息、裂解特征和液相色谱保留时间，从唐古特大黄的甲醇提取物中初步鉴定出30种成分。其中，7种化合物首次从唐古特大黄中被描述，2种从大黄属中首次被描述。这里使用的分析工具对于快速分离和鉴定唐古特大黄甲醇提取物中的多种微量成分很有价值。
