虎杖叶化学成分及其超高效液相色谱-电喷雾串联四极杆飞行时间质谱联用分析研究
Study on Chemical Constituents in Polygoni Cuspidati Folium and its Preparation by UPLC-ESI-Q-TOF-MS/MS.
作者信息
Wang Xin, Qin Yao, Li Guang-Quan, Chen Shuai, Ma Jing-Qi, Guo Yan-Lei, Luo Wei-Zao
机构信息
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, No. 1166 Liutai Avenue, Wenjiang District, Chengdu 610075, China.
Chongqing Institute of Chinese Materia Medica, Chongqing, China.
出版信息
J Chromatogr Sci. 2018 May 1;56(5):425-435. doi: 10.1093/chromsci/bmy017.
A rapid and credible analytical method was developed using online UPLC-ESI-Q-TOF-MS/MS to identify chemical constituents in Polygoni cuspidati folium and its preparation. By accurate mass measurements within 6.5 ppm error for [M-H]- ion in routine analysis, 26 chemical constituents, including tannin, derivatives of phenylpropionic acid, stilbene, flavonoid, anthraquinone, torachryson and its derivatives, were identified or tentatively characterized. Among them, five constituents (compounds 19-23) were firstly reported in Polygoni cuspidati folium, other 17 constituents were coexisting in both Polygoni cuspidati folium and its preparation. Fragmentation behaviors of different categories of constituents were also investigated to confirm the results. This established UPLC-ESI-Q-TOF-MS/MS method, with reliance and efficiency for the identification the major constituents, would be the basis for quality control of Polygoni cuspidati folium and its preparation.
采用在线超高效液相色谱-电喷雾电离-四极杆-飞行时间串联质谱（UPLC-ESI-Q-TOF-MS/MS）建立了一种快速可靠的分析方法，用于鉴定虎杖叶及其制剂中的化学成分。通过在常规分析中对[M-H]-离子进行误差在6.5 ppm以内的精确质量测量，鉴定或初步表征了26种化学成分，包括单宁、苯丙酸衍生物、二苯乙烯、黄酮类、蒽醌、托拉曲松及其衍生物。其中，5种成分（化合物19 - 23）首次在虎杖叶中报道，另外17种成分在虎杖叶及其制剂中均有共存。还研究了不同类别成分的裂解行为以确证结果。所建立的UPLC-ESI-Q-TOF-MS/MS方法对主要成分的鉴定具有可靠性和高效性，将为虎杖叶及其制剂的质量控制奠定基础。
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