Key Laboratory for Utility of Environment-Friendly Composite Materials and Biomass, Universities of Gansu Province, Lanzhou 730000, China.
China-Malaysia National Joint Laboratory, Biomedical Research Center, Northwest MinZu University, Lanzhou 730000, China.
Molecules. 2022 Aug 19;27(16):5291. doi: 10.3390/molecules27165291.
The abuse of agricultural antibiotics has led to the emergence of drug-resistant phytopathogens. Rifampicin and streptomycin and streptomycin resistance subsp. (S1) was obtained from pathological plants in a previous experiment. , derived from the Chinese plateau area, exhibits excellent antibacterial activity against S1, yet the action mode has not been fully understood. In present text, the cell wall integrity of the S1 was tested by the variation of the cellular proteins, SDS polyacrylamide gel electrophoresis (SDS-PAGE), scanning electron microscopy (SEM) and Fourier transform infrared spectrophotometer (FTIR) characteristics. Label-free quantitative proteomics was further used to identify the DEPs in the pathogen response to treatment with Maxim. ex Balf. extract (abbreviated as RTMBE). Based on the bioinformatics analysis of these different expressed proteins (DEPs), RTMBE mainly inhibited some key protein expressions of beta-Lactam resistance, a two-component system and phosphotransferase system. Most of these membrane proteins were extraordinarily suppressed, which was also consistent with the morphological tests. In addition, from the downregulated flagellar motility related proteins, it was also speculated that RTMBE played an essential antibacterial role by affecting the swimming motility of the cells. The results indicated that can be used to attenuate the virulence of the drug-resistant phytopathogenic bacteria.
农业抗生素的滥用导致了抗药性植物病原体的出现。在之前的实验中，从病态植物中获得了利福平（rifampicin）和链霉素（streptomycin）以及链霉素抗性亚种（S1）。从中国高原地区分离出的 对 S1 表现出优异的抗菌活性，但作用模式尚未完全了解。在本文中，通过细胞蛋白质的变化、SDS 聚丙烯酰胺凝胶电泳（SDS-PAGE）、扫描电子显微镜（SEM）和傅里叶变换红外光谱仪（FTIR）特征来测试 S1 的细胞壁完整性。进一步使用无标记定量蛋白质组学来鉴定病原菌对 Maxim. ex Balf. 提取物（简称 RTMBE）治疗的响应中的差异表达蛋白（DEPs）。基于这些不同表达蛋白（DEPs）的生物信息学分析，RTMBE 主要抑制了一些关键蛋白的表达，如β-内酰胺抗性、双组分系统和磷酸转移酶系统。这些膜蛋白大部分被异常抑制，这与形态学测试结果一致。此外，从下调的鞭毛运动相关蛋白中推测，RTMBE 通过影响细胞的泳动运动来发挥重要的抗菌作用。结果表明， 可用于减轻耐药植物病原菌的毒力。
