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II型毒素-抗毒素系统基因在（16M）（B19）中的持留菌形成及表达

Persister cells formation and expression of type II Toxin-Antitoxin system genes in (16M) (B19).

作者信息

Amraei Fatemeh, Narimisa Negar, Sadeghi Kalani Behrooz, Lohrasbi Vahid, Masjedian Jazi Faramarz

机构信息

Microbial Biotechnology Research Center, Iran University of Medical Science, Tehran, Iran.

Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Pathol. 2020 Spring;15(2):127-133. doi: 10.30699/ijp.2020.118902.2294. Epub 2020 Feb 19.

DOI:10.30699/ijp.2020.118902.2294
PMID:32215028
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7081757/
Abstract

BACKGROUND & OBJECTIVE: Persister cells are defined as a subpopulation of bacteria that are capable of reducing their metabolism and switching to dormancy in stress conditions. Persister cells formation has been attributed to numerous mechanisms, including stringent response and Toxin-Antitoxin (TA) systems. This study aimed to investigate the hypothetical role of TA systems in persister cells formation of strains by evaluating toxins of type II TA systems (, , , cogT) expression.

METHODS

strains treated with a lethal dose of gentamicin and ampicillin and to determine the number of surviving cells, bacterial colonies were counted at different time intervals. The role of TA systems in persister cell formation was then determined by toxin expression levels using qRT- PCR method.

RESULTS

Our results showed the viability of persister cells after 7 h. The results of relative qRT- PCR showed higher levels of toxin gene expression due to stress conditions, suggesting the possible role of TA systems in persister cells formation and antibiotics tolerance.

CONCLUSION

The results of this study showed that considering the importance of persistence and the tolerance to antibiotics, further studies on persister cells formation and related genes such as the TA system genes in strains might help us to identify the precise mechanisms leading to persister cells formation.

摘要

背景与目的

持留菌被定义为细菌的一个亚群，它们能够在应激条件下降低代谢并进入休眠状态。持留菌的形成归因于多种机制，包括严谨反应和毒素 - 抗毒素（TA）系统。本研究旨在通过评估II型TA系统（、、、cogT）毒素的表达，探讨TA系统在菌株持留菌形成中的假设作用。

方法

用致死剂量的庆大霉素和氨苄西林处理菌株，为确定存活细胞数量，在不同时间间隔对细菌菌落进行计数。然后使用qRT - PCR方法通过毒素表达水平确定TA系统在持留菌形成中的作用。

结果

我们的结果显示持留菌在7小时后仍具有活力。相对qRT - PCR结果显示，由于应激条件，毒素基因表达水平较高，这表明TA系统在持留菌形成和抗生素耐受性中可能发挥作用。

结论

本研究结果表明，考虑到持留性和抗生素耐受性的重要性，对菌株中持留菌形成及相关基因（如TA系统基因）的进一步研究可能有助于我们确定导致持留菌形成的精确机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f13/7081757/71b066d67a04/ijp-15-127-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f13/7081757/e13b8a55b14c/ijp-15-127-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f13/7081757/68aa4eced617/ijp-15-127-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f13/7081757/71b066d67a04/ijp-15-127-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f13/7081757/e13b8a55b14c/ijp-15-127-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f13/7081757/68aa4eced617/ijp-15-127-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f13/7081757/71b066d67a04/ijp-15-127-g003.jpg

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