Shiri Mariam, Komaki Alireza, Oryan Shahrbanoo, Taheri Masoumeh, Komaki Hamidreza, Etaee Farshid
a Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
b Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Can J Physiol Pharmacol. 2017 Apr;95(4):382-387. doi: 10.1139/cjpp-2016-0274. Epub 2016 Nov 2.
Despite previous findings on the effects of cannabinoid and vanilloid systems on learning and memory, the effects of the combined stimulation of these 2 systems on learning and memory have not been studied. Therefore, in this study, we tested the interactive effects of cannabinoid and vanilloid systems on learning and memory in rats by using passive avoidance learning (PAL) tests. Forty male Wistar rats were divided into the following 4 groups: (1) control (DMSO+saline), (2) WIN55,212-2, (3) capsaicin, and (4) WIN55,212-2 + capsaicin. On test day, capsaicin, a vanilloid receptor type 1 (TRPV1) agonist, or WIN55,212-2, a cannabinoid receptor (CB/CB) agonist, or both substances were injected intraperitoneally. Compared to the control group, the group treated with capsaicin (TRPV1 agonist) had better scores in the PAL acquisition and retention test, whereas treatment with WIN55,212-2 (CB/CB agonist) decreased the test scores. Capsaicin partly reduced the effects of WIN55,212-2 on PAL and memory. We conclude that the acute administration of a TRPV1 agonist improves the rats' cognitive performance in PAL tasks and that a vanilloid-related mechanism may underlie the agonistic effect of WIN55,212-2 on learning and memory.
尽管之前已有关于大麻素系统和香草酸受体系统对学习和记忆影响的研究结果，但这两种系统联合刺激对学习和记忆的影响尚未得到研究。因此，在本研究中，我们通过被动回避学习（PAL）测试，检验了大麻素系统和香草酸受体系统对大鼠学习和记忆的交互作用。40只雄性Wistar大鼠被分为以下4组：（1）对照组（二甲基亚砜+生理盐水），（2）WIN55,212-2组，（3）辣椒素组，（4）WIN55,212-2+辣椒素组。在测试当天，腹腔注射辣椒素（一种1型香草酸受体（TRPV1）激动剂）、WIN55,212-2（一种大麻素受体（CB/CB）激动剂）或两种物质。与对照组相比，用辣椒素（TRPV1激动剂）处理的组在PAL获取和保留测试中得分更高，而用WIN55,212-2（CB/CB激动剂）处理则降低了测试得分。辣椒素部分减轻了WIN55,212-2对PAL和记忆的影响。我们得出结论，急性给予TRPV1激动剂可改善大鼠在PAL任务中的认知表现，并且香草酸受体相关机制可能是WIN55,212-2对学习和记忆产生激动作用的基础。
