• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

接触抑制阶段miR-29a/b/c表达下调通过靶向DNMT3A促进3T3-L1脂肪生成。

Downregulated miR-29a/b/c during Contact Inhibition Stage Promote 3T3-L1 Adipogenesis by Targeting DNMT3A.

作者信息

Zhu Yingjie, Zheng Guangyong, Wang Huichao, Jia Yudong, Zhang Ying, Tang Yanfeng, Li Wenlong, Fan Yanan, Zhang Xiaodong, Liu Youwen, Liu Sanhong

机构信息

Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.

CAS-MPG Partner Institute for Computational Biology, Chinese Academy of Sciences, Shanghai, China.

出版信息

PLoS One. 2017 Jan 23;12(1):e0170636. doi: 10.1371/journal.pone.0170636. eCollection 2017.

DOI:10.1371/journal.pone.0170636
PMID:28114345
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5256892/
Abstract

Differentiation of 3T3-L1 cells into adipocytes involves a highly-orchestrated series of events including contact inhibition (CI), clonal expansion, growth arrest, and terminal differentiation. Recent study demonstrated that 3T3-L1 preadipocytes will not be differentiated into mature adipocytes without CI stage, which indicated that CI stage plays an important role during 3T3-L1 adipogenesis. However, the molecular mechanism is not yet fully understood. In the present study, we found that the expression level of miR-29a/b/c was decreased and the expression of DNMT3A was up-regulated during CI stage, respectively. Furthermore, overexpression of miR-29a/b/c during CI stage inhibits adipogenesis significantly but not at other stages. In addition, miR-29a/b/c repressed DNMT3A expression by directly targeting its 3' untranslated region (3' UTR). Our data reveal a novel mechanism of miR-29a/b/c in the regulation of adipogenesis.

摘要

3T3-L1细胞向脂肪细胞的分化涉及一系列高度协调的事件，包括接触抑制（CI）、克隆扩增、生长停滞和终末分化。最近的研究表明，3T3-L1前脂肪细胞如果没有CI阶段就不会分化为成熟脂肪细胞，这表明CI阶段在3T3-L1脂肪生成过程中起着重要作用。然而，其分子机制尚未完全阐明。在本研究中，我们发现miR-29a/b/c的表达水平在CI阶段分别降低，而DNMT3A的表达上调。此外，在CI阶段过表达miR-29a/b/c可显著抑制脂肪生成，但在其他阶段则不然。另外，miR-29a/b/c通过直接靶向DNMT3A的3'非翻译区（3'UTR）来抑制其表达。我们的数据揭示了miR-29a/b/c调控脂肪生成的一种新机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/e94e6d3682c5/pone.0170636.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/2962cf41eed1/pone.0170636.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/77f5186b874e/pone.0170636.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/399a07e75e04/pone.0170636.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/4cb96b4db832/pone.0170636.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/5c5e03b606df/pone.0170636.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/e94e6d3682c5/pone.0170636.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/2962cf41eed1/pone.0170636.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/77f5186b874e/pone.0170636.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/399a07e75e04/pone.0170636.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/4cb96b4db832/pone.0170636.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/5c5e03b606df/pone.0170636.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a576/5256892/e94e6d3682c5/pone.0170636.g006.jpg

相似文献

1
Downregulated miR-29a/b/c during Contact Inhibition Stage Promote 3T3-L1 Adipogenesis by Targeting DNMT3A.接触抑制阶段miR-29a/b/c表达下调通过靶向DNMT3A促进3T3-L1脂肪生成。
PLoS One. 2017 Jan 23;12(1):e0170636. doi: 10.1371/journal.pone.0170636. eCollection 2017.
2
MiR-185 inhibits 3T3-L1 cell differentiation by targeting SREBP-1.微小RNA-185通过靶向固醇调节元件结合蛋白-1抑制3T3-L1细胞分化。
Biosci Biotechnol Biochem. 2017 Sep;81(9):1747-1754. doi: 10.1080/09168451.2017.1347485. Epub 2017 Jul 13.
3
PU.1 promotes miR-191 to inhibit adipogenesis in 3T3-L1 preadipocytes.PU.1 促进 miR-191 抑制 3T3-L1 前脂肪细胞的脂肪生成。
Biochem Biophys Res Commun. 2014 Aug 22;451(2):329-33. doi: 10.1016/j.bbrc.2014.07.130. Epub 2014 Aug 2.
4
miR-206-3p Inhibits 3T3-L1 Cell Adipogenesis via the c-Met/PI3K/Akt Pathway.miR-206-3p通过c-Met/PI3K/Akt通路抑制3T3-L1细胞脂肪生成。
Int J Mol Sci. 2017 Jul 14;18(7):1510. doi: 10.3390/ijms18071510.
5
miR-135a-5p inhibits 3T3-L1 adipogenesis through activation of canonical Wnt/β-catenin signaling.miR-135a-5p 通过激活经典 Wnt/β-连环蛋白信号通路抑制 3T3-L1 脂肪生成。
J Mol Endocrinol. 2014 Jun;52(3):311-20. doi: 10.1530/JME-14-0013.
6
Characterization of miR-210 in 3T3-L1 adipogenesis.miR-210 在 3T3-L1 脂肪生成中的特征分析。
J Cell Biochem. 2013 Dec;114(12):2699-707. doi: 10.1002/jcb.24617.
7
miR-709 inhibits 3T3-L1 cell differentiation by targeting GSK3β of Wnt/β-catenin signaling.微小RNA-709通过靶向Wnt/β-连环蛋白信号通路的糖原合成酶激酶3β来抑制3T3-L1细胞分化。
Cell Signal. 2014 Nov;26(11):2583-9. doi: 10.1016/j.cellsig.2014.07.017. Epub 2014 Jul 16.
8
Epigenetic programming of Dnmt3a mediated by AP2α is required for granting preadipocyte the ability to differentiate.AP2α介导的Dnmt3a表观遗传编程是赋予前脂肪细胞分化能力所必需的。
Cell Death Dis. 2016 Dec 1;7(12):e2496. doi: 10.1038/cddis.2016.378.
9
TNFα-induced up-regulation of miR-155 inhibits adipogenesis by down-regulating early adipogenic transcription factors.TNFα 诱导的 miR-155 上调抑制脂肪生成，通过下调早期脂肪生成转录因子。
Biochem Biophys Res Commun. 2011 Oct 28;414(3):618-24. doi: 10.1016/j.bbrc.2011.09.131. Epub 2011 Oct 2.
10
MicroRNA-344 inhibits 3T3-L1 cell differentiation via targeting GSK3β of Wnt/β-catenin signaling pathway.miRNA-344 通过靶向 Wnt/β-连环蛋白信号通路中的 GSK3β 抑制 3T3-L1 细胞分化。
FEBS Lett. 2014 Jan 31;588(3):429-35. doi: 10.1016/j.febslet.2013.12.002. Epub 2013 Dec 10.

引用本文的文献

1
miR-344d-3p regulates osteogenic and adipogenic differentiation of mouse mandibular bone marrow mesenchymal stem cells.miR-344d-3p 调控小鼠下颌骨髓间充质干细胞的成骨和成脂分化。
PeerJ. 2023 Feb 14;11:e14838. doi: 10.7717/peerj.14838. eCollection 2023.
2
Mindfulness intervention improves cognitive function in older adults by enhancing the level of miRNA-29c in neuron-derived extracellular vesicles.正念干预通过提高神经元衍生细胞外囊泡中 miRNA-29c 的水平来改善老年人的认知功能。
Sci Rep. 2021 Nov 8;11(1):21848. doi: 10.1038/s41598-021-01318-y.
3
Expression and prognosis analysis of family in acute myeloid leukemia.

本文引用的文献

1
MicroRNAs as regulators of metabolic disease: pathophysiologic significance and emerging role as biomarkers and therapeutics.微小RNA作为代谢性疾病的调节因子：病理生理意义以及作为生物标志物和治疗手段的新作用
Int J Obes (Lond). 2016 Jan;40(1):88-101. doi: 10.1038/ijo.2015.170. Epub 2015 Aug 27.
2
MicroRNA regulatory networks in human adipose tissue and obesity.人类脂肪组织和肥胖中的 microRNA 调控网络。
Nat Rev Endocrinol. 2015 May;11(5):276-88. doi: 10.1038/nrendo.2015.25. Epub 2015 Mar 3.
3
Regulation of Adipocyte Differentiation via MicroRNAs.
家族在急性髓系白血病中的表达与预后分析。
Aging (Albany NY). 2020 Jun 26;12(14):14677-14690. doi: 10.18632/aging.103520.
4
miR-30a-3p inhibits the proliferation of liver cancer cells by targeting DNMT3a through the PI3K/AKT signaling pathway.微小RNA-30a-3p通过PI3K/AKT信号通路靶向DNA甲基转移酶3a来抑制肝癌细胞的增殖。
Oncol Lett. 2020 Jan;19(1):606-614. doi: 10.3892/ol.2019.11179. Epub 2019 Dec 3.
5
MicroRNA Microarray Profiling in Infantile Hemangiomas.婴儿血管瘤中的微小RNA微阵列分析
Eplasty. 2019 Apr 16;19:e13. eCollection 2019.
通过 microRNAs 调节脂肪细胞分化。
Endocrinol Metab (Seoul). 2014 Jun;29(2):122-35. doi: 10.3803/EnM.2014.29.2.122.
4
miR-27 inhibits adipocyte differentiation via suppressing CREB expression.微小RNA-27通过抑制CREB表达来抑制脂肪细胞分化。
Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):590-6. doi: 10.1093/abbs/gmu036. Epub 2014 Jun 5.
5
MicroRNAs: emerging roles in adipogenesis and obesity.微小RNA：在脂肪生成和肥胖症中的新作用
Cell Signal. 2014 Sep;26(9):1888-96. doi: 10.1016/j.cellsig.2014.05.006. Epub 2014 May 15.
6
let-7 enhances osteogenesis and bone formation while repressing adipogenesis of human stromal/mesenchymal stem cells by regulating HMGA2.Let-7 通过调控 HMGA2 增强人基质/间充质干细胞的成骨作用和骨形成，同时抑制其脂肪生成。
Stem Cells Dev. 2014 Jul 1;23(13):1452-63. doi: 10.1089/scd.2013.0600. Epub 2014 May 22.
7
The physiological and pathophysiological roles of adipocyte miRNAs.脂肪细胞 miRNAs 的生理和病理生理作用。
Biochem Cell Biol. 2013 Aug;91(4):195-202. doi: 10.1139/bcb-2012-0053. Epub 2013 Jan 25.
8
Early leptin blockade predisposes fat-fed rats to overweight and modifies hypothalamic microRNAs.早期瘦素阻断使高脂喂养的大鼠易患超重，并改变下丘脑 microRNAs。
J Endocrinol. 2013 Jun 1;218(1):35-47. doi: 10.1530/JOE-12-0561. Print 2013 Jul.
9
miR-17-5p and miR-106a are involved in the balance between osteogenic and adipogenic differentiation of adipose-derived mesenchymal stem cells.miR-17-5p和miR-106a参与脂肪来源间充质干细胞成骨分化与成脂分化之间的平衡。
Stem Cell Res. 2013 May;10(3):313-24. doi: 10.1016/j.scr.2012.11.007. Epub 2012 Dec 3.
10
A novel regulator of macrophage activation: miR-223 in obesity-associated adipose tissue inflammation.肥胖相关脂肪组织炎症中巨噬细胞激活的新型调节因子：miR-223
Circulation. 2012 Jun 12;125(23):2892-903. doi: 10.1161/CIRCULATIONAHA.111.087817. Epub 2012 May 11.