Schultz Brent Earl, Spock Christopher R, Tom Laura K, Kong Yong, Canadas Karina T, Kim Samuel, Waner Milton, O Teresa, Antaya Richard, Narayan Deepak
Yale School of Medicine, New Haven, Conn.
Yale School of Public Health: Biostatistics, New Haven, Conn.
Eplasty. 2019 Apr 16;19:e13. eCollection 2019.
MicroRNAs are short, noncoding RNA molecules that negatively regulate the stability and translational efficiency of target mRNAs. They are critical regulators of growth and development. Our aim was to identify microRNAs involved in the growth and regulation of infantile hemangiomas. In addition, we searched for the presence of Piwi-interacting RNAs in hemangioma tissue as another regulator of infantile hemangiomas. RNA was extracted from hemangioma specimens from 3 clinical, age-based categories: proliferative (N = 16), quiescent (N = 8), and involuting (N = 9). RNAs from human dermal microvascular endothelial cells were used as controls. MicroRNA microarray was performed, and the expression profiles of the hemangiomas and endothelial cells were compared using the test. 5' End-labeling of RNA of our hemangioma specimens was performed for Piwi-interacting RNA detection. Analysis confirmed statistically significant downregulated (N = 18) and upregulated (N = 15) microRNAs. Piwi-interacting RNA analysis did not detect Piwi-interacting RNA transcripts in the hemangioma specimens. The differential expression of microRNAs found in our hemangioma specimens provides insight into the regulation of hemangioma formation and proliferation, quiescence, and fibrofatty involution. Piwi-interacting RNA transcripts were not detected in the hemangioma specimens. These novel findings will help in establishing new therapeutic and diagnostic initiatives for these tumors.
微小RNA是短的非编码RNA分子，可负向调节靶mRNA的稳定性和翻译效率。它们是生长和发育的关键调节因子。我们的目的是鉴定参与婴儿血管瘤生长和调节的微小RNA。此外，我们在血管瘤组织中寻找与Piwi相互作用RNA的存在，作为婴儿血管瘤的另一种调节因子。从3个基于年龄的临床类别（增殖期，N = 16；静止期，N = 8；消退期，N = 9）的血管瘤标本中提取RNA。来自人真皮微血管内皮细胞的RNA用作对照。进行微小RNA微阵列分析，并使用检验比较血管瘤和内皮细胞的表达谱。对我们的血管瘤标本的RNA进行5'末端标记以检测与Piwi相互作用的RNA。分析证实了微小RNA在统计学上有显著下调（N = 18）和上调（N = 15）。与Piwi相互作用RNA分析未在血管瘤标本中检测到与Piwi相互作用的RNA转录本。在我们的血管瘤标本中发现的微小RNA的差异表达为血管瘤形成、增殖、静止和纤维脂肪消退的调节提供了见解。在血管瘤标本中未检测到与Piwi相互作用的RNA转录本。这些新发现将有助于为这些肿瘤建立新的治疗和诊断方案。
