State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing 100085, China; Sino-Danish Center for Education and Research, Beijing 100190, China.
Department of Science and Environment, Roskilde University, P.O. Box 260, 4000 Roskilde, Denmark.
J Environ Sci (China). 2017 Jan;51:138-145. doi: 10.1016/j.jes.2016.08.015. Epub 2016 Sep 27.
With the development of nanotechnology, gold (Au) and graphene oxide (GO) nanoparticles have been widely used in various fields, resulting in an increased release of these particles into the environment. The released nanoparticles may eventually accumulate in sediment, causing possible ecotoxicological effects to benthic invertebrates. However, the impact of Au-NPs and GO-NPs on the cosmopolitan oligochaete, Tubifex tubifex, in sediment exposure is not known. Mortality, behavioral impact (GO-NP and Au-NP) and uptake (only Au-NP) of sediment-associated Au-NPs (4.9±0.14nm) and GO-NPs (116±0.05nm) to T. tubifex were assessed in a number of 5-day exposure experiments. The results showed that the applied Au-NP concentrations (10 and 60μg Au/g dry weight sediment) had no adverse effect on T. tubifex survival, while Au bioaccumulation increased with exposure concentration. In the case of GO-NPs, no mortality of T. tubifex was observed at a concentration range of 20 and 180μg GO/g dry weight sediment, whereas burrowing activity was significantly reduced at 20 and 180μg GO/g dry weight sediment. Our results suggest that Au-NPs at 60μg Au/g or GO-NPs at 20 and 180μg GO/g were detected by T. tubifex as toxicants during short-term exposures.
随着纳米技术的发展，金（Au）和氧化石墨烯（GO）纳米粒子已被广泛应用于各个领域，导致这些粒子越来越多地释放到环境中。释放的纳米粒子最终可能会在沉积物中积累，对底栖无脊椎动物造成潜在的生态毒理学影响。然而，Au-NPs 和 GO-NPs 对世界性寡毛类环节动物，如颤蚓（Tubifex tubifex）在沉积物暴露中的影响尚不清楚。在一系列为期 5 天的暴露实验中，评估了 T. tubifex 对与沉积物结合的 Au-NPs（4.9±0.14nm）和 GO-NPs（116±0.05nm）的死亡率、行为影响（GO-NP 和 Au-NP）和摄取（仅 Au-NP）。结果表明，所应用的 Au-NP 浓度（10 和 60μg Au/g 干重沉积物）对 T. tubifex 的存活率没有不良影响，而 Au 的生物积累随着暴露浓度的增加而增加。对于 GO-NPs，在 20 和 180μg GO/g 干重沉积物浓度范围内，T. tubifex 没有观察到死亡率，而在 20 和 180μg GO/g 干重沉积物浓度范围内，其挖掘活动明显减少。我们的研究结果表明，在短期暴露期间，T. tubifex 将 60μg Au/g 的 Au-NPs 或 20 和 180μg GO/g 的 GO-NPs 视为有毒物质。
