Moradi Pouya, Keyvani Hossein, Javad Mousavi Seyed-Ali, Karbalaie Niya Mohammad Hadi, Esghaei Maryam, Bokharaei-Salim Farah, Ataei-Pirkooh Angila, Monavari Seyed Hamidreza
Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Microb Pathog. 2017 Mar;104:171-174. doi: 10.1016/j.micpath.2017.01.030. Epub 2017 Jan 20.
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease, which can be lethal with chronic complications. Viral infections may be associated with IPF and other fibrotic lung diseases. In the present study, we investigate for the first time in Iran the related viral etiology of IPF in order to detect three respiratory viruses; human adenovirus, enterovirus and bocavirus.
In this cross-sectional study which was supported by Iran University of Medical Sciences, Tehran, Iran. The diagnostic criteria for IPF were based on internationally accepted clinical and imaging criteria in accordance with the 2011 IPF guidelines. 30 nasopharyngeal (NP) swabs or broncho-alveolar lavage (BAL) samples were obtained from the lung of IPF patients that were diagnosed by a sophisticated practitioner from April 2015 to February 2016. Real-time (RT) polymerase chain reaction (PCR) method was performed to detect the three viruses. Fluorescence dye of a labeled probe recorded the results in order to create positive and negative controls. SPSS version 20 software was used to calculate basic descriptive and frequency features.
Of 30 specimens, 13 (43.4%) were male and 17 (56.6%) were female with the total mean age ± standard deviation 68.2 ± 12.0. RT-PCR assay results illustrated there was no infection of human adenovirus, enterovirus, and bocavirus detected in these samples. Significant results between IPF incidence and variables were not significant (p > 0.05).
The causes of IPF in Iranian patients need more research although, based on the results of this study, there was no association between human adenovirus, enterovirus, bocavirus, and IPF.
特发性肺纤维化（IPF）是一种进行性肺部疾病，可因慢性并发症而致命。病毒感染可能与IPF及其他纤维化肺病有关。在本研究中，我们首次在伊朗调查IPF的相关病毒病因，以检测三种呼吸道病毒；人腺病毒、肠道病毒和博卡病毒。
本横断面研究由伊朗德黑兰医科大学资助。IPF的诊断标准基于2011年IPF指南中国际认可的临床和影像学标准。2015年4月至2016年2月期间，从经经验丰富的医生诊断为IPF的患者肺部获取30份鼻咽（NP）拭子或支气管肺泡灌洗（BAL）样本。采用实时（RT）聚合酶链反应（PCR）方法检测这三种病毒。用标记探针的荧光染料记录结果，以建立阳性和阴性对照。使用SPSS 20版软件计算基本描述性和频率特征。
30份标本中，男性13例（43.4%），女性17例（56.6%），总平均年龄±标准差为68.2±12.0。RT-PCR检测结果表明，这些样本中未检测到人类腺病毒、肠道病毒和博卡病毒感染。IPF发病率与各变量之间的显著结果不显著（p>0.05）。
伊朗患者IPF的病因需要更多研究，尽管基于本研究结果，人类腺病毒、肠道病毒、博卡病毒与IPF之间无关联。
