Meng Qingyang, Hu Xiaoqing, Huang Hongjie, Liu Zhenlong, Yuan Lan, Shao Zhenxing, Jiang Yanfang, Zhang Jiying, Fu Xin, Duan Xiaoning, Ao Yingfang
Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
Medical and Healthy Analysis Centre, Peking University, 38 Xueyuan Road, Haidian District, Beijing 100191, People's Republic of China.
Acta Biomater. 2017 Apr 15;53:279-292. doi: 10.1016/j.actbio.2017.01.055. Epub 2017 Jan 20.
Due to avascular and hypocellular nature of cartilage, repair of articular cartilage defects within synovial joints still poses a significant clinical challenge. To promote neocartilage properties, we established a functional scaffold named APM-E7 by conjugating a bone marrow-derived mesenchymal stem cell (BM-MSC) affinity peptide (E7) onto the acellular peritoneum matrix (APM). During in vitro culture, the APM-E7 scaffold can support better proliferation as well as better differentiation into chondrocytes of BM-MSCs. After implanting into cartilage defects in rabbits for 24weeks, compared with microfracture and APM groups, the APM-E7 scaffolds exhibited superior quality of neocartilage without transplant rejection, according to general observations, histological assessment, synovial fluid analysis, magnetic resonance imaging (MRI) and nanomechanical properties. This APM-E7 scaffold provided a scaffold for cell attachment, which was crucial for cartilage regeneration. Overall, the APM-E7 is a promising biomaterial with low immunogenicity for one-step cartilage repair by promoting autologous connective tissue progenitor (CTP) attachment.
We report the one-step transplantation of functional acellular peritoneum matrix (APM-E7) with specific mesenchymal stem cell recruitment to repair rabbit cartilage injury. The experimental results illustrated that the APM-E7 scaffold was successfully fabricated, which could specifically recruit MSCs and fill the cartilage defects in the femoral trochlear of rabbits at 24weeks post-surgery. The repaired tissue was hyaline cartilage, which exhibited ideal mechanical stability. The APM-E7 biomaterial could provide scaffold for MSCs and improve cell homing, which are two key factors required for cartilage tissue engineering, thereby providing new insights into cartilage tissue engineering.
由于软骨的无血管和低细胞特性，滑膜关节内关节软骨缺损的修复仍然是一项重大的临床挑战。为了促进新软骨特性，我们通过将骨髓间充质干细胞（BM-MSC）亲和肽（E7）与脱细胞腹膜基质（APM）结合，建立了一种名为APM-E7的功能性支架。在体外培养过程中，APM-E7支架可以更好地支持BM-MSC的增殖以及向软骨细胞的分化。将其植入兔软骨缺损24周后，根据一般观察、组织学评估、滑液分析、磁共振成像（MRI）和纳米力学性能，与微骨折组和APM组相比，APM-E7支架显示出更高质量的新软骨，且无移植排斥反应。这种APM-E7支架为细胞附着提供了支架，这对软骨再生至关重要。总体而言，APM-E7是一种有前景的生物材料，具有低免疫原性，可通过促进自体结缔组织祖细胞（CTP）附着来一步修复软骨。
我们报告了功能性脱细胞腹膜基质（APM-E7）的一步移植，其具有特定的间充质干细胞募集能力，用于修复兔软骨损伤。实验结果表明，成功制备了APM-E7支架，其可以特异性募集间充质干细胞，并在术后24周填充兔股骨滑车的软骨缺损。修复后的组织为透明软骨，表现出理想的机械稳定性。APM-E7生物材料可为间充质干细胞提供支架并改善细胞归巢，这是软骨组织工程所需的两个关键因素，从而为软骨组织工程提供了新的见解。
