改良琼脂平板培养物中[具体物质]的检测改进
Improved Detection of in Modified Agar Plate Cultures.
作者信息
Pocaterra Leonor A, Ferrara Giuseppe, Peñaranda Rosaura, Rojas Elsy, Pérez-Chacón Gladymar, Hernán Aurora, Certad Gabriela, Goldstein Carlos, Núñez Luz
机构信息
Cátedra de Parasitología, Escuela de Medicina "José María Vargas," Universidad Central de Venezuela, Caracas, Venezuela.
Laboratorio de Parasitosis Intestinales, Escuela de Medicina "José María Vargas," Universidad Central de Venezuela, Caracas, Venezuela.
出版信息
Am J Trop Med Hyg. 2017 Apr;96(4):863-865. doi: 10.4269/ajtmh.16-0414. Epub 2017 Jan 23.
AbstractA modification of Koga agar plate culture was performed, consisting of a 2 × 2-cm cellophane paper centered on the agar plate to prevent bacterial contamination of the agar and daily dish examinations (days 2-5). Between January 2000 and July 2005, we examined 1,708 infection-suspected patients, of which 147 (8.6%) harbored . Single modified agar plate cultures exhibited superior sensitivity (93.2%), compared with different three-sample screening methods (sensitivity-Baermann: 76.6%, formalin-ethyl acetate: 22%, and direct smear: 15.3%). Agar plate cultures stand out as helpful alternatives for improved detection and therapy monitoring in poor countries and endemic areas. Combined with Baermann methods, they provide increased probability for detection.
摘要
对古贺琼脂平板培养法进行了改良，即在琼脂平板中央放置一张2×2厘米的玻璃纸，以防止琼脂受到细菌污染，并进行每日平板检查（第2至5天）。2000年1月至2005年7月期间，我们检查了1708例疑似感染患者，其中147例（8.6%）携带[此处原文缺失具体内容]。与不同的三种样本筛查方法（敏感性——贝曼氏法：76.6%，甲醛-乙酸乙酯法：22%，直接涂片法：15.3%）相比，单一改良琼脂平板培养法显示出更高的敏感性（93.2%）。琼脂平板培养法是贫困国家和流行地区改善检测和治疗监测的有益替代方法。与贝曼氏法结合使用时，它们提高了检测[此处原文缺失具体内容]的概率。
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