Mathematical Model for Predicting the Growth Probability of in Combinations of NaCl and NaNO under Aerobic or Evacuated Storage Conditions.

The objective of this study was to describe the growth patterns of in combinations of NaCl and NaNO, using a probabilistic model. A mixture of strains (NCCP10826, ATCC13565, ATCC14458, ATCC23235, and ATCC27664) was inoculated into nutrient broth plus NaCl (0, 0.25, 0.5, 0.75, 1, 1.25, 1.5, and 1.75%) and NaNO (0, 15, 30, 45, 60, 75, 90, 105, and 120 ppm). The samples were then incubated at 4, 7, 10, 12 and 15℃ for up to 60 d under aerobic or vacuum conditions. Growth responses [growth (1) or no growth (0)] were then determined every 24 h by turbidity, and analyzed to select significant parameters (<0.05) by a stepwise selection method, resulting in a probabilistic model. The developed models were then validated with observed growth responses. growth was observed only under aerobic storage at 10-15℃. At 10-15℃, NaCl and NaNO did not inhibit growth at less than 1.25% NaCl. Concentration dependency was observed for NaCl at more than 1.25%, but not for NaNO. The concordance percentage between observed and predicted growth data was approximately 93.86%. This result indicates that growth can be inhibited in vacuum packaging and even aerobic storage below 10℃. Furthermore, NaNO does not effectively inhibit growth.