Avcioglu Nermin Hande, Bilkay Isil Seyis
Hacettepe University, Faculty of Science, Department of Biology (Biotechnology), Beytepe, TR-06800 Ankara, Turkey.
Food Technol Biotechnol. 2016 Dec;54(4):450-454. doi: 10.17113/ftb.54.04.16.4518.
In this study, optimization conditions for cyanide biodegradation by strain were determined to be 25 °C, pH=7 and 150 rpm at the concentration of 0.5 mM potassium cyanide in the medium. Additionally, it was found that strain is not only able to degrade potassium cyanide, but also to degrade potassium hexacyanoferrate(II) trihydrate and sodium ferrocyanide decahydrate with the efficiencies of 85 and 87.5%, respectively. Furthermore, this strain degraded potassium cyanide in the presence of different ions such as magnesium, nickel, cobalt, iron, chromium, arsenic and zinc, in variable concentrations (0.1, 0.25 and 0.5 mM) and as a result the amount of the bacteria in the biodegradation media decreased with the increase of ion concentration. Lastly, it was also observed that sterile crude extract of strain degraded potassium cyanide on the fifth day of incubation. Based on these results, it is concluded that both culture and sterile crude extract of will be used in cyanide removal from different wastes.
在本研究中，确定该菌株生物降解氰化物的优化条件为：在培养基中氰化钾浓度为0.5 mM时，温度25 °C、pH = 7、转速150 rpm。此外，还发现该菌株不仅能够降解氰化钾，还能降解三水合亚铁氰化钾和十水合亚铁氰化钠，降解效率分别为85%和87.5%。此外，该菌株在不同离子（镁、镍、钴、铁、铬、砷和锌）存在的情况下，以不同浓度（0.1、0.25和0.5 mM）降解氰化钾，结果生物降解培养基中的细菌数量随着离子浓度的增加而减少。最后，还观察到该菌株的无菌粗提物在培养的第五天降解了氰化钾。基于这些结果，可以得出结论，该菌株的培养物和无菌粗提物都将用于从不同废物中去除氰化物。
