出生后发育过程中大鼠黑质中单胺及两种形式单胺氧化酶的测定
Determination of monoamines and both forms of monoamine oxidase in the rat's substantia nigra during postnatal development.
作者信息
Venero J L, Santiago M, Machado A, Cano J
机构信息
Departamento de Bioquímica, Facultad de Farmacia, Sevilla, Spain.
出版信息
Life Sci. 1989;45(14):1277-83. doi: 10.1016/0024-3205(89)90130-6.
Changes in biogenic amine content in the substantia nigra and in both forms of monoamine oxidase in substantia nigra and striatum of the rat during postnatal development (15-180 days) have been studied. Dopamine and serotonin had the same levels at day 15, however, each monoamine showed a different developmental profile. Dopamine levels and their metabolites (except 3-methoxytyramine) decreased during postnatal development. Serotonin levels and their main metabolite, 5-hydroxyindolacetic acid, underwent an increase during all stages studied. There were no statistically significant changes in noradrenaline levels until day 180 when they increased with respect to day 15. The highest activity of the monoamine oxidase-A in substantia nigra coincided with the highest 5-hydroxyindolacetic acid:serotonin ratio. Monoamine oxidase-A in the striatum did not change contrary to that which happened in substantia nigra. The monoamine oxidase-B:monoamine oxidase-A ratio increased during development both in the substantia nigra and the striatum. The significance of these changes is discussed.
研究了大鼠出生后发育阶段（15 - 180天）黑质中生物胺含量以及黑质和纹状体中两种单胺氧化酶的变化。多巴胺和5-羟色胺在第15天时水平相同，然而，每种单胺呈现出不同的发育模式。多巴胺水平及其代谢产物（除3-甲氧基酪胺外）在出生后发育过程中下降。5-羟色胺水平及其主要代谢产物5-羟吲哚乙酸在所有研究阶段均升高。去甲肾上腺素水平直到第180天才有统计学显著变化，相对于第15天有所升高。黑质中单胺氧化酶A的最高活性与最高的5-羟吲哚乙酸:5-羟色胺比值一致。纹状体中的单胺氧化酶A没有变化，与黑质中的情况相反。黑质和纹状体中，单胺氧化酶B:单胺氧化酶A的比值在发育过程中均升高。讨论了这些变化的意义。
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