NADPH Oxidases and Measurement of Reactive Oxygen Species.

The NADPH oxidase (Nox) family of enzymes is expressed in many tissues that are involved in hypertension, including blood vessels, kidney, and brain. In these tissues, the products of NADPH oxidase activity, superoxide and ultimately hydrogen peroxide, act as intracellular and extracellular messengers during compartmentalized cellular signaling. The correct measurement of Nox activity and its products is crucial to enable studies of how these signaling pathways affect the molecular mechanisms underlying hypertension. Here, we describe methods for detection and measurement of hydrogen peroxide and superoxide derived from NADPH oxidases in biological samples such as cells and tissues.