用于测量NADPH氧化酶活性的探针和检测方法的最新进展
Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases.
作者信息
Zielonka Jacek, Hardy Micael, Michalski Radosław, Sikora Adam, Zielonka Monika, Cheng Gang, Ouari Olivier, Podsiadły Radosław, Kalyanaraman Balaraman
机构信息
Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA.
Aix Marseille Univ, CNRS, ICR, 13013, Marseille, France.
出版信息
Cell Biochem Biophys. 2017 Dec;75(3-4):335-349. doi: 10.1007/s12013-017-0813-6. Epub 2017 Jun 29.
Abstract
NADPH oxidases are a family of enzymes capable of transferring electrons from NADPH to molecular oxygen. A major function of NADPH oxidases is the activation of molecular oxygen into reactive oxygen species. Increased activity of NADPH oxidases has been implicated in various pathologies, including cardiovascular disease, neurological dysfunction, and cancer. Thus, NADPH oxidases have been identified as a viable target for the development of novel therapeutics exhibiting inhibitory effects on NADPH oxidases. Here, we describe the development of new assays for measuring the activity of NADPH oxidases enabling the high-throughput screening for NADPH oxidase inhibitors.
摘要
NADPH氧化酶是一类能够将电子从NADPH转移至分子氧的酶。NADPH氧化酶的一个主要功能是将分子氧激活为活性氧。NADPH氧化酶活性增加与多种病理状况有关，包括心血管疾病、神经功能障碍和癌症。因此，NADPH氧化酶已被确定为开发对NADPH氧化酶具有抑制作用的新型治疗药物的可行靶点。在此，我们描述了用于测量NADPH氧化酶活性的新检测方法的开发，该方法能够对NADPH氧化酶抑制剂进行高通量筛选。
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