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Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation.通过二氢乙锭氧化的高效液相色谱分析测定超氧化物生成量和NADPH氧化酶活性。
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2
Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays.在膜分析中，细胞色素P450酶而非NADPH氧化酶是NADPH依赖的光泽精化学发光的来源。
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A Critical Review of Methodologies to Detect Reactive Oxygen and Nitrogen Species Stimulated by NADPH Oxidase Enzymes: Implications in Pesticide Toxicity.对检测由NADPH氧化酶刺激产生的活性氧和氮物种的方法的批判性综述：对农药毒性的影响
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Detection and characterisation of radicals using electron paramagnetic resonance (EPR) spin trapping and related methods.利用电子顺磁共振（EPR）自旋捕集及相关方法检测和表征自由基。
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用于测量NADPH氧化酶活性的探针和检测方法的最新进展

Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases.

作者信息

Zielonka Jacek, Hardy Micael, Michalski Radosław, Sikora Adam, Zielonka Monika, Cheng Gang, Ouari Olivier, Podsiadły Radosław, Kalyanaraman Balaraman

机构信息

Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA.

Aix Marseille Univ, CNRS, ICR, 13013, Marseille, France.

出版信息

Cell Biochem Biophys. 2017 Dec;75(3-4):335-349. doi: 10.1007/s12013-017-0813-6. Epub 2017 Jun 29.

DOI:10.1007/s12013-017-0813-6
PMID:28660426
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5693611/
Abstract

NADPH oxidases are a family of enzymes capable of transferring electrons from NADPH to molecular oxygen. A major function of NADPH oxidases is the activation of molecular oxygen into reactive oxygen species. Increased activity of NADPH oxidases has been implicated in various pathologies, including cardiovascular disease, neurological dysfunction, and cancer. Thus, NADPH oxidases have been identified as a viable target for the development of novel therapeutics exhibiting inhibitory effects on NADPH oxidases. Here, we describe the development of new assays for measuring the activity of NADPH oxidases enabling the high-throughput screening for NADPH oxidase inhibitors.

摘要

NADPH氧化酶是一类能够将电子从NADPH转移至分子氧的酶。NADPH氧化酶的一个主要功能是将分子氧激活为活性氧。NADPH氧化酶活性增加与多种病理状况有关，包括心血管疾病、神经功能障碍和癌症。因此，NADPH氧化酶已被确定为开发对NADPH氧化酶具有抑制作用的新型治疗药物的可行靶点。在此，我们描述了用于测量NADPH氧化酶活性的新检测方法的开发，该方法能够对NADPH氧化酶抑制剂进行高通量筛选。