通过流式细胞术分离和鉴定循环微颗粒
Isolation and Characterization of Circulating Microparticles by Flow Cytometry.
作者信息
Burger Dylan, Oleynik Paul
机构信息
Kidney Research Centre, Ottawa Hospital Research Institute, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada, K1H 8M5.
Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.
出版信息
Methods Mol Biol. 2017;1527:271-281. doi: 10.1007/978-1-4939-6625-7_21.
Microparticles are small fragments (0.1-1.0 μm) of cellular membrane which are shed by cells under conditions of stress. As levels of circulating microparticles are elevated in disease, there has been significant interest in their assessment and quantification under pathological conditions. Here we describe a protocol for the isolation of microparticles from plasma samples and their characterization/quantification by flow cytometry. This assay has been employed for the assessment of microparticle levels in both human and animal plasma and may also be modified for the characterization of microparticles from culture media or from other biological samples (i.e., urine).
微粒是细胞膜的小碎片（0.1 - 1.0μm），在应激条件下由细胞脱落。由于疾病状态下循环微粒水平会升高，因此人们对其在病理条件下的评估和定量产生了浓厚兴趣。在此，我们描述了一种从血浆样本中分离微粒并通过流式细胞术对其进行表征/定量的方法。该检测方法已用于评估人和动物血浆中的微粒水平，也可进行修改以用于表征来自培养基或其他生物样本（如尿液）的微粒。
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