Gusel'nikova T V, Belov A P, Gradova N B
Mikrobiologiia. 1989 Mar-Apr;58(2):202-5.
The work was aimed at studying the effect of yeast autolysate on the content and subcellular distribution of free amino acids in yeast cells. The overall pool of free amino acids decreased 1.5-2 times when mineral nitrogen in the growth medium was substituted either completely or partly by yeast autolysate. As was shown using the technique of differential extraction, the vacuolar pool of the cell is mainly responsible for the decrease in the content of free amino acids.
这项工作旨在研究酵母自溶产物对酵母细胞中游离氨基酸含量及亚细胞分布的影响。当生长培养基中的无机氮全部或部分被酵母自溶产物替代时，游离氨基酸的总量下降了1.5至2倍。采用差异提取技术的研究结果表明，细胞液泡中的氨基酸库是游离氨基酸含量下降的主要原因。
