小Y染色体DNA片段在微芯片电泳上的异常分离
Anomalous Separation of Small Y-Chromosomal DNA Fragments on Microchip Electrophoresis.
作者信息
Jabasini Mohammad, Ewis Ashraf, Sato Youichi, Nakahori Yutaka, Baba Yoshinobu
机构信息
Department of Pharmaceutical Information Science, Institute of Health Biosciences, The University of Tokushima, 1-78-1 Sho-machi, 770-8505 Tokushima, Japan.
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603 Nagoya, Japan.
出版信息
Sci Pharm. 2016 May 26;84(3):507-513. doi: 10.3390/scipharm84030507.
We investigated an anomalous DNA separation where two DNA fragments from the human Y-chromosome sY638 (64 bp) and sY592 (65 bp), with only one base pair difference, were separated. This result is abnormal since in a previous study, we found that 5 bp was the minimum difference between two DNA fragments that the microchip electrophoresis system can separate. The formation of a mini-loop in the structure of the DNA fragment of sY638 (64 bp) was strongly expected to be the reason. To investigate this, we synthesized three modified DNA fragments for sY638 (64 bp), and the modifications were in two expected locations for possible mini-loop formation. Later, the separation between sY592 (65 bp) and the three modified fragments of sY638 (64 bp) was not possible. Thus, we conclude that the formation of a mini-loop in the structure of the DNA is the reason behind this anomalous separation.
我们研究了一种异常的DNA分离现象，其中来自人类Y染色体的两个DNA片段sY638（64 bp）和sY592（65 bp），仅相差一个碱基对，却被分离了。这一结果是异常的，因为在之前的一项研究中，我们发现微芯片电泳系统能够分离的两个DNA片段之间的最小差异是5 bp。强烈推测sY638（64 bp）的DNA片段结构中形成了一个小环是导致这一现象的原因。为了对此进行研究，我们合成了三个针对sY638（64 bp）的修饰DNA片段，修饰位于可能形成小环的两个预期位置。后来，sY592（65 bp）与sY638（64 bp）的三个修饰片段之间无法分离。因此，我们得出结论，DNA结构中形成小环是这种异常分离背后的原因。
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