Grzechnik Pawel, Tan-Wong Sue Mei, Proudfoot Nick J
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Trends Biochem Sci. 2014 Jul;39(7):319-27. doi: 10.1016/j.tibs.2014.05.001. Epub 2014 Jun 10.
Bidirectional promoters are a common feature of many eukaryotic organisms from yeast to humans. RNA Polymerase II that is recruited to this type of promoter can start transcribing in either direction using alternative DNA strands as the template. Such promiscuous transcription can lead to the synthesis of unwanted transcripts that may have negative effects on gene expression. Recent studies have identified transcription termination and gene looping as critical players in the enforcement of promoter directionality. Interestingly, both mechanisms share key components. Here, we focus on recent findings relating to the transcriptional output of bidirectional promoters.
双向启动子是从酵母到人类等许多真核生物的共同特征。被招募到这类启动子的RNA聚合酶II可以使用替代DNA链作为模板，在任一方向上开始转录。这种杂乱的转录可能导致合成不需要的转录本，这些转录本可能对基因表达产生负面影响。最近的研究已经确定转录终止和基因环化是促进启动子方向性的关键因素。有趣的是，这两种机制共享关键成分。在这里，我们重点关注与双向启动子转录输出相关的最新发现。
