基于氧化锌的微流控 pH 传感器：一种快速识别血液中循环肿瘤细胞的通用方法。

ZnO-Based Microfluidic pH Sensor: A Versatile Approach for Quick Recognition of Circulating Tumor Cells in Blood.

机构信息

Micro/Nano Technology Center, ‡Graduate School of Science and Technology, §Department of Mechanical Engineering, and ∥Department of Precision Engineering, Tokai University , 4-1-1 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan.

出版信息

ACS Appl Mater Interfaces. 2017 Feb 15;9(6):5193-5203. doi: 10.1021/acsami.6b16261. Epub 2017 Feb 3.

DOI:10.1021/acsami.6b16261

PMID:28117972

Abstract

The present study is concerned about the development of highly sensitive and stable microfluidic pH sensor for possible identification of circulating tumor cells (CTCs) in blood. The precise pH measurements between silver-silver chloride (Ag/AgCl) reference electrode and zinc oxide (ZnO) working electrode have been investigated in the microfluidic device. Since there is a direct link between pH and cancer cells, the developed device is one of the valuable tools to examine circulating tumor cells (CTCs) in blood. The ZnO-based working electrode was deposited by radio frequency (rf) sputtering technique. The potential voltage difference between the working and reference electrodes (Ag/AgCl) is evaluated on the microfluidic device. The ideal Nernstian response of -43.71165 mV/pH was achieved along with high stability and quick response time. Finally, to evaluate the real time capability of the developed microfluidic device, in vitro testing was done with A549, A7r5, and MDCK cells.

摘要

本研究关注于开发高度灵敏和稳定的微流控 pH 传感器，以用于在血液中可能识别循环肿瘤细胞（CTC）。在微流控装置中，已经研究了银-氯化银（Ag/AgCl）参比电极和氧化锌（ZnO）工作电极之间的精确 pH 测量。由于 pH 值与癌细胞之间存在直接联系，因此所开发的设备是用于检查血液中循环肿瘤细胞（CTC）的有价值工具之一。基于 ZnO 的工作电极通过射频（rf）溅射技术沉积。在微流控装置上评估工作电极和参比电极（Ag/AgCl）之间的电位电压差。实现了理想的 Nernstian 响应，为-43.71165 mV/pH，同时具有高稳定性和快速响应时间。最后，为了评估所开发的微流控装置的实时性能，使用 A549、A7r5 和 MDCK 细胞进行了体外测试。