McCann Conor, Gilpin Victoria, Scott Cameron, Pourshahidi L Kirsty, Gill Chris I R, Davis James
School of Engineering, Ulster University, Belfast, Northern Ireland.
School of Biomedical Sciences, Ulster University, Coleraine, Northern Ireland.
J Mater Sci. 2023;58(35):14207-14219. doi: 10.1007/s10853-023-08881-x. Epub 2023 Sep 8.
The development of a 3D printed sensor for direct incorporation within stoma pouches is described. Laser induced graphene scribed on either side of polyimide film served as the basis of a 2 electrode configuration that could be integrated within a disposable pouch sensor for the periodic monitoring of ileostomy fluid pH. The graphene sensors were characterised using electron microscopy, Raman spectroscopy, DekTak profilometry with the electrochemical properties investigated using both cyclic and square wave voltammetry. Adsorbed riboflavin was employed as a biocompatible redox probe for the voltammetric measurement of pH. The variation in peak position with pH was found to be linear over pH 3-8 with a sub Nernstian response (43 mV/pH). The adsorbed probe was found to be reversible and exhibited minimal leaching through repeated scanning. The performance of the system was assessed in a heterogeneous bacterial fermentation mixture simulating ileostomy fluid with the pH recorded before and after 96 h incubation. The peak profile in the bacterial medium provided an unambiguous signal free from interference with the calculated pH before and after incubation (pH 5.3 to 3.66) in good agreement with that obtained with commercial pH probes.
The online version contains supplementary material available at 10.1007/s10853-023-08881-x.
本文描述了一种用于直接集成到造口袋中的3D打印传感器的开发。在聚酰亚胺薄膜两侧通过激光诱导刻写的石墨烯构成了一种双电极配置的基础，该配置可集成到一次性袋式传感器中，用于定期监测回肠造口液的pH值。使用电子显微镜、拉曼光谱、Dektak表面轮廓仪对石墨烯传感器进行了表征，并通过循环伏安法和方波伏安法研究了其电化学性质。吸附的核黄素被用作生物相容性氧化还原探针，用于pH值的伏安测量。发现在pH值3-8范围内，峰位置随pH值的变化呈线性，具有亚能斯特响应（43 mV/pH）。发现吸附的探针是可逆的，并且通过重复扫描显示出最小的浸出。在模拟回肠造口液的异质细菌发酵混合物中评估了该系统的性能，记录了96小时孵育前后的pH值。细菌培养基中的峰轮廓提供了一个明确的信号，不受干扰，孵育前后计算的pH值（从pH 5.3到3.66）与使用商业pH探针获得的值高度一致。
在线版本包含可在10.1007/s10853-023-08881-x获取的补充材料。
