Uplekar Swapna, Rao Pavitra Nagesh, Ramanathapuram Lalitha, Awasthi Vikky, Verma Kalpana, Sutton Patrick, Ali Syed Zeeshan, Patel Ankita, G Sri Lakshmi Priya, Ravishankaran Sangamithra, Desai Nisha, Tandel Nikunj, Choubey Sandhya, Barla Punam, Kanagaraj Deena, Eapen Alex, Pradhan Khageswar, Singh Ranvir, Jain Aarti, Felgner Philip L, Davies D Huw, Carlton Jane M, Das Jyoti
Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, United States of America.
National Institute of Malaria Research, Indian Council of Medical Research, Sector 8, Dwarka, New Delhi, India.
PLoS Negl Trop Dis. 2017 Jan 24;11(1):e0005323. doi: 10.1371/journal.pntd.0005323. eCollection 2017 Jan.
Understanding naturally acquired immune responses to Plasmodium in India is key to improving malaria surveillance and diagnostic tools. Here we describe serological profiling of immune responses at three sites in India by probing protein microarrays consisting of 515 Plasmodium vivax and 500 Plasmodium falciparum proteins with 353 plasma samples. A total of 236 malaria-positive (symptomatic and asymptomatic) plasma samples and 117 malaria-negative samples were collected at three field sites in Raurkela, Nadiad, and Chennai. Indian samples showed significant seroreactivity to 265 P. vivax and 373 P. falciparum antigens, but overall seroreactivity to P. vivax antigens was lower compared to P. falciparum antigens. We identified the most immunogenic antigens of both Plasmodium species that were recognized at all three sites in India, as well as P. falciparum antigens that were associated with asymptomatic malaria. This is the first genome-scale analysis of serological responses to the two major species of malaria parasite in India. The range of immune responses characterized in different endemic settings argues for targeted surveillance approaches tailored to the diverse epidemiology of malaria across the world.
了解印度对疟原虫的自然获得性免疫反应是改善疟疾监测和诊断工具的关键。在此，我们通过用353份血浆样本探测由515种间日疟原虫蛋白和500种恶性疟原虫蛋白组成的蛋白质微阵列，描述了印度三个地点的免疫反应血清学概况。在劳尔克拉、纳迪亚德和金奈的三个实地地点共收集了236份疟疾阳性（有症状和无症状）血浆样本和117份疟疾阴性样本。印度样本对265种间日疟原虫抗原和373种恶性疟原虫抗原表现出显著的血清反应性，但与恶性疟原虫抗原相比，对间日疟原虫抗原的总体血清反应性较低。我们确定了在印度所有三个地点都能识别的两种疟原虫的最具免疫原性的抗原，以及与无症状疟疾相关的恶性疟原虫抗原。这是印度首次对两种主要疟原虫的血清学反应进行全基因组规模分析。在不同流行地区所表征的免疫反应范围表明，需要针对世界各地疟疾的不同流行病学情况采取有针对性的监测方法。
