巴西大西洋森林中新热带灵长类动物针对抗原的IgM抗体反应。
IgM antibody responses against antigens in neotropical primates in the Brazilian Atlantic Forest.
作者信息
de Assis Gabriela Maíra Pereira, de Alvarenga Denise Anete Madureira, Souza Luisa Braga E, Sánchez-Arcila Juan Camilo, Silva Eduardo Fernandes E, de Pina-Costa Anielle, Gonçalves Gustavo Henrique Pereira, Souza Júlio César de Junior, Nunes Ana Julia Dutra, Pissinatti Alcides, Moreira Silvia Bahadian, Torres Leticia de Menezes, Costa Helena Lott, Tinoco Herlandes da Penha, Pereira Valéria do Socorro, Soares Irene da Silva, de Sousa Taís Nóbrega, Ntumngia Francis Babila, Adams John H, Kano Flora Satiko, Hirano Zelinda Maria Braga, Pratt-Riccio Lilian Rose, Daniel-Ribeiro Cláudio Tadeu, Ferreira Joseli Oliveira, Carvalho Luzia Helena, Alves de Brito Cristiana Ferreira
机构信息
Grupo de Pesquisa em Biologia Molecular e Imunologia da malária, Instituto René Rachou/Fiocruz Minas, Belo Horizonte, Brazil.
School of Natural Sciences, Molecular and Cell Biology Department, University of California, Merced, Merced, CA, United States.
出版信息
Front Cell Infect Microbiol. 2023 Sep 27;13:1169552. doi: 10.3389/fcimb.2023.1169552. eCollection 2023.
INTRODUCTION
Zoonotic transmission is a challenge for the control and elimination of malaria. It has been recorded in the Atlantic Forest, outside the Amazon which is the endemic region in Brazil. However, only very few studies have assessed the antibody response, especially of IgM antibodies, in Neotropical primates (NP). Therefore, in order to contribute to a better understanding of the immune response in different hosts and facilitate the identification of potential reservoirs, in this study, naturally acquired IgM antibody responses against Plasmodium antigens were evaluated, for the first time, in NP from the Atlantic Forest.
METHODS
The study was carried out using 154 NP samples from three different areas of the Atlantic Forest. IgM antibodies against peptides of the circumsporozoite protein (CSP) from different species and different erythrocytic stage antigens were detected by ELISA.
RESULTS
Fifty-nine percent of NP had IgM antibodies against at least one CSP peptide and 87% against at least one vivax erythrocytic stage antigen. Levels of antibodies against PvAMA-1 were the highest compared to the other antigens. All families of NP showed IgM antibodies against CSP peptides, and, most strikingly, against erythrocytic stage antigens. Generalized linear models demonstrated that IgM positivity against PvCSP and PvAMA-1 was associated with PCR-detectable blood-stage malaria infection and the host being free-living. Interestingly, animals with IgM against both PvCSP and PvAMA-1 were 4.7 times more likely to be PCR positive than animals that did not have IgM for these two antigens simultaneously.
DISCUSSION
IgM antibodies against different spp. antigens are present in NP from the Atlantic Forest. High seroprevalence and antibody levels against blood-stage antigens were observed, which had a significant association with molecular evidence of infection. IgM antibodies against CSP and AMA-1 may be used as a potential marker for the identification of NP infected with Plasmodium, which are reservoirs of malaria in the Brazilian Atlantic Forest.
引言
人畜共患传播是疟疾控制和消除工作面临的一项挑战。在巴西疟疾流行地区亚马逊以外的大西洋森林中已有相关记录。然而，仅有极少数研究评估了新热带灵长类动物（NP）的抗体反应，尤其是IgM抗体反应。因此，为了有助于更好地理解不同宿主的免疫反应并促进潜在宿主的识别，本研究首次对来自大西洋森林的NP的自然获得性抗疟原虫抗原IgM抗体反应进行了评估。
方法
本研究使用了来自大西洋森林三个不同区域的154份NP样本。通过酶联免疫吸附测定法（ELISA）检测针对不同物种环子孢子蛋白（CSP）肽段以及不同红细胞阶段抗原的IgM抗体。
结果
59%的NP具有针对至少一种CSP肽段的IgM抗体，87%具有针对至少一种间日疟原虫红细胞阶段抗原的IgM抗体。与其他抗原相比，针对间日疟原虫裂殖体表面蛋白1（PvAMA-1）的抗体水平最高。所有NP家族均显示出针对CSP肽段的IgM抗体，最显著的是，针对红细胞阶段抗原的IgM抗体。广义线性模型表明，针对间日疟原虫CSP（PvCSP）和PvAMA-1的IgM阳性与PCR可检测到的血液阶段疟疾感染以及宿主为自由生活状态有关。有趣的是，同时具有针对PvCSP和PvAMA-1的IgM的动物PCR检测呈阳性的可能性是不同时具有这两种抗原IgM的动物的4.7倍。
讨论
来自大西洋森林的NP中存在针对不同疟原虫物种抗原的IgM抗体。观察到针对血液阶段抗原的高血清阳性率和抗体水平，这与感染的分子证据存在显著关联。针对CSP和AMA-1的IgM抗体可作为识别感染疟原虫的NP的潜在标志物，这些NP是巴西大西洋森林中的疟疾宿主。
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