O'Neill S C, Valdeolmillos M, Smith G L, Eisner D A
Department of Physiology, University College London, UK.
Mol Cell Biochem. 1989 Sep 7;89(2):199-203. doi: 10.1007/BF00220776.
We have investigated the effects of inhibiting aerobic and/or anaerobic metabolism on contraction, intracellular calcium and pH in single rat ventricular myocytes. Inhibition of aerobic metabolism alone (with CN) had little effect on these variables. However, if anaerobic glycolysis was also inhibited, then the application of CN decreased systolic [Ca2+]i and increased diastolic [Ca2+]i. There was also a development of a diastolic contracture which lagged behind the increase of diastolic [Ca2+]i. These events were accompanied by an intracellular acidosis. The acidosis was shown to depress contraction and perhaps to account for the fact that diastolic [Ca2+]i increased before the contracture.
我们研究了抑制有氧和/或无氧代谢对单个大鼠心室肌细胞收缩、细胞内钙和pH值的影响。单独抑制有氧代谢（使用氰化物）对这些变量几乎没有影响。然而，如果无氧糖酵解也受到抑制，那么应用氰化物会降低收缩期细胞内钙离子浓度（[Ca2+]i）并增加舒张期[Ca2+]i。还出现了舒张期挛缩，其滞后于舒张期[Ca2+]i的增加。这些事件伴随着细胞内酸中毒。酸中毒被证明会抑制收缩，并且可能解释了舒张期[Ca2+]i在挛缩之前增加的原因。
