College of Basic Medical Science, Hebei University , Baoding 071000, China.
Affiliated Hospital of Hebei University , Baoding 071000, China.
ACS Appl Mater Interfaces. 2017 Feb 22;9(7):5784-5792. doi: 10.1021/acsami.6b14682. Epub 2017 Feb 9.
Europium-doped GdO nanotubes (GdO:Eu NTs) have been extensively applied in the field of bioscience for their photostability and magnetic properties. Nevertheless, the distribution and interaction between GdO:Eu NTs and metabolism of bone are not yet sufficiently understood. In this study, a systematic study of the toxicity and distribution of GdO:Eu NTs in mice after oral administration was carried out. The results showed that a small number of the GdO:Eu NTs could pass through biological barriers into the lung, liver, and spleen, but a high concentration was observed in bone. Furthermore, the effects of GdO:Eu NTs on bone metabolism were systematically studied in vitro and in vivo when accumulating in bone. After being administered to mice, the GdO:Eu NTs extremely enhanced the bone mineral density and bone biomechanics. In vitro the GdO:Eu NTs increased the alkaline phosphatase (ALP) activity and mineralization and promoted the expression of osteogenesis genes in preosteoblasts MC3T3-E1 through activation of the BMP signaling pathway. This study will be significant for appropriate application of GdO:Eu NTs in the biomedical field and expounding the molecular mechanism of bone metabolism.
掺铕氧化钆纳米管（GdO:Eu NTs）因其光稳定性和磁性而在生物科学领域得到了广泛应用。然而，GdO:Eu NTs 与骨代谢的分布和相互作用尚不完全清楚。在这项研究中，我们对口服 GdO:Eu NTs 后其在小鼠体内的毒性和分布进行了系统研究。结果表明，少量的 GdO:Eu NTs 可以通过生物屏障进入肺、肝和脾，但在骨中观察到高浓度。此外，我们还系统研究了 GdO:Eu NTs 在骨内蓄积时对骨代谢的影响。在给予小鼠后，GdO:Eu NTs 极大地提高了骨密度和骨生物力学性能。体外实验中，GdO:Eu NTs 通过激活 BMP 信号通路，增加碱性磷酸酶（ALP）活性和矿化，并促进成骨前体细胞 MC3T3-E1 中骨生成基因的表达。这项研究对于 GdO:Eu NTs 在生物医学领域的合理应用以及阐明骨代谢的分子机制具有重要意义。
