Yin Y, Wada O, Manabe S
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Mutat Res. 1989 Nov;215(1):107-13. doi: 10.1016/0027-5107(89)90223-6.
In order to determine a suitable indicator for estimating the exposure levels of the dietary carcinogen 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1), a carcinogenic glutamic acid pyrolysis product, the levels of Glu-P-1 bound to blood components were monitored for 8 weeks by a high-performance liquid chromatography method after the dietary carcinogen was administered as single oral doses (0.2-50 mg) to rabbits. In all rabbits dosed with Glu-P-1, Glu-P-1 in erythrocytes was detectable even on day 42 after administration. Glu-P-1 in plasma disappeared faster than did Glu-P-1 in erythrocytes. Glu-P-1 levels in rabbit hemoglobin were linearly related to administered doses at all points of time investigated. The results suggest that Glu-P-1 covalently bound to hemoglobin is very suitable for monitoring long-term exposure levels.
为了确定一种合适的指标来估算膳食致癌物2-氨基-6-甲基二吡啶并[1,2-a:3',2'-d]咪唑（Glu-P-1，一种致癌性谷氨酸热解产物）的暴露水平，在给兔子单次口服膳食致癌物（0.2 - 50毫克）后，采用高效液相色谱法对与血液成分结合的Glu-P-1水平进行了8周的监测。在所有给予Glu-P-1的兔子中，即使在给药后第42天，红细胞中的Glu-P-1仍可检测到。血浆中的Glu-P-1比红细胞中的Glu-P-1消失得更快。在所研究的所有时间点，兔血红蛋白中的Glu-P-1水平与给药剂量呈线性相关。结果表明，与血红蛋白共价结合的Glu-P-1非常适合用于监测长期暴露水平。
