Kanai Y, Manabe S, Wada O
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Mutat Res. 1988 Feb;207(2):63-7. doi: 10.1016/0165-7992(88)90043-7.
In order to confirm in vivo N-acetylation of 2-amino-6-methyl-dipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) in rats, we developed a new high-performance liquid chromatography (HPLC) method for detecting the N-acetyl derivative of Glu-P-1 in animal organs. Using this method, N-acetyl-Glu-P-1 was detected in rat liver, kidney and intestinal contents 6 h after intraperitoneal injection of Glu-P-1. This fact provides evidence to support in vivo N-acetylation of Glu-P-1 in rats.
为了证实大鼠体内2-氨基-6-甲基-二吡啶并[1,2-a:3',2'-d]咪唑（Glu-P-1）的N-乙酰化作用，我们开发了一种新的高效液相色谱（HPLC）方法，用于检测动物器官中Glu-P-1的N-乙酰衍生物。采用该方法，在腹腔注射Glu-P-1 6小时后，在大鼠肝脏、肾脏和肠道内容物中检测到了N-乙酰-Glu-P-1。这一事实为支持大鼠体内Glu-P-1的N-乙酰化作用提供了证据。
