Huimin Guo, Xiaoying Fu, Hongwei Du, Wei Cong, Xiangcai Meng
Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Int J Pharm Investig. 2016 Oct-Dec;6(4):225-230. doi: 10.4103/2230-973X.195932.
The quality of radix scutellariae is particularly associated with environmental stresses, but detailed mechanisms remained unclear. Plant under unfavorable situation generates redundant reactive oxygen species (ROS), and ROS can modify the secondary metabolism. The varied quality of radix scutellariae could be explained by ROS.
.004, 0.4, and 40 μmol/L of sodium dithionite (NaSO), a material producing ROS, were applied to to mimic unfavorable situation. The relationship between ROS, antioxidant enzymes activity, and secondary metabolite was investigated.
ROS level fails to rise due to both the antioxidase and the secondary metabolites. The activities of both superoxide dismutase and catalase in the roots of showed a moderately improvement, meanwhile the phenylalanine ammonia lyase was strongly expressed, and the biosynthesis of flavonoids was heavily elevated. Although the glycosides such as baicalin and wogonoside changed little, the aglycones with the highest effective, such as baicalein and wogonin, were increased by approximately 50%-100%.
This is very valuable in insight into the stress physiology and provides a strong tool to enhance the quality of radix scutellariae.
黄芩的品质尤其与环境胁迫相关，但具体机制仍不清楚。植物在不利环境下会产生过量的活性氧（ROS），而ROS会改变次生代谢。黄芩品质的差异可能可用ROS来解释。
将产生ROS的物质连二亚硫酸钠（NaSO）以0.004、0.4和40 μmol/L的浓度施用于黄芩以模拟不利环境。研究了ROS、抗氧化酶活性和次生代谢产物之间的关系。
由于抗氧化酶和次生代谢产物的作用，ROS水平未能升高。黄芩根中超氧化物歧化酶和过氧化氢酶的活性均有适度提高，同时苯丙氨酸解氨酶强烈表达，黄酮类化合物的生物合成大幅增加。虽然黄芩苷和汉黄芩苷等糖苷变化不大，但活性最高的苷元如黄芩素和汉黄芩素增加了约50%-100%。
这对于深入了解胁迫生理学非常有价值，并为提高黄芩品质提供了有力工具。
