Fu Xiao-Ying, Guo Hui-Min, Cong Wei, Meng Xiang-Cai
Heilongjiang University of Chinese Medicine， Harbin 150040, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jan;43(2):271-287. doi: 10.19540/j.cnki.cjcmm.20171030.009.
The increasing demand of Chinese materia medica could not be supplied by wild resource, and the cultivated medicinal materials become popular, which led to decreased quality of many medicinal materials due to the difference of the circumstance between the wild and the cultivated. How to improve quality becomes key points of Chinese medicine resource. The leaves of Scutellaria baicalensis were sprayed with H₂O₂, the activities of superoxide dismutase (SOD) and catalase (CAT) changed little, but there had been a marked decrease of peroxidase (POD) and ascorbic oxidase (APX), which showed that the antioxidase system declined. Meanwhile, H₂O₂, as enhanced the expression of phenylalnine ammonialyase (PAL) and β-glucuronidase (GUS) as well as activity of PAL, promoted the biosynthesis and biotransformation of flavonoids. At the day 2 after treated, H₂O₂ of 0.004 μmol·L⁻¹ the contents of the baicalin and the wogonoside decreased slightly, but the contents of the baicalein and the wogonin increased significantly, the baicalein from 0.094% to 0.324%, the wogonin from 0.060% to 0.110%, i. e. increased 246% and 83.3%, respectively.
野生资源已无法满足对中药材日益增长的需求，人工栽培的药材开始流行起来，但由于野生环境与人工栽培环境的差异，许多药材的质量有所下降。如何提高质量成为中药资源的关键问题。用H₂O₂喷施黄芩叶片后，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性变化不大，但过氧化物酶（POD）和抗坏血酸氧化酶（APX）的活性显著降低，表明抗氧化酶系统功能衰退。同时，H₂O₂增强了苯丙氨酸解氨酶（PAL）和β-葡萄糖醛酸酶（GUS）的表达以及PAL的活性，促进了黄酮类化合物的生物合成和生物转化。处理后第2天，0.004 μmol·L⁻¹的H₂O₂使黄芩苷和汉黄芩苷的含量略有下降，但黄芩素和汉黄芩素的含量显著增加，黄芩素从0.094%增至0.324%，汉黄芩素从0.060%增至0.110%，即分别增加了246%和83.3%。
