Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.
Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
Talanta. 2019 Jan 15;192:169-174. doi: 10.1016/j.talanta.2018.09.042. Epub 2018 Sep 14.
A sensitive tool for simultaneous quantitative determination of two analytes in a single spot with the use of a bioimprinted protein is presented for the first time. BSA is chosen as a scaffold for generation of binding sites specific towards two compounds. A multiplex immunosorbent assay for screening of two cereal-born mycotoxins, deoxynivalenol and zearalenone, in wheat and maize is realized with the use of fluorescent silica coated quantum dots as labels. Water-soluble fluorescent nanostructures consist of core/shell Cd-QDs enrobed in silica shells to ensure their solubility. The mycotoxins are simultaneously detected by scanning the assay outcome at two different wavelengths, since two QD@SiO labelled conjugates fluorescent in different parts of the spectrum. The assay is combined with a rapid extraction technique. The limits of detection for the simultaneous determination were 100 and 700 µg kg in both matrices for zearalenone and deoxynivalenol, respectively. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the obtained results.
本文首次提出了一种使用生物印迹蛋白在单个斑点中同时定量测定两种分析物的灵敏工具。BSA 被选为生成针对两种化合物的结合位点的支架。使用荧光硅涂层量子点作为标记物，实现了用于筛选小麦和玉米中两种谷物来源的霉菌毒素，脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的多重免疫吸附测定法。水溶性荧光纳米结构由核/壳 Cd-QDs 包裹在硅壳中组成，以确保其溶解度。通过在两个不同波长下扫描测定结果，同时检测两种 QD@SiO 标记的缀合物在光谱的不同部分发出荧光。该测定法与快速提取技术相结合。对于同时测定，在两种基质中的检测限分别为 100 和 700 µg kg-1，用于玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇。液相色谱-串联质谱（LC-MS/MS）用于确认获得的结果。
