Corbillé Anne-Gaëlle, Preterre Cécile, Rolli-Derkinderen Malvyne, Coron Emmanuel, Neunlist Michel, Lebouvier Thibaud, Derkinderen Pascal
Inserm, U913, Nantes F-44035, France; Nantes University, Nantes F-44035, France; CHU Nantes, Department of Neurology, Nantes F-44093, France.
Inserm, U913, Nantes F-44035, France; Nantes University, Nantes F-44035, France; CHU Nantes, Institut des Maladies de l'Appareil Digestif, Nantes F-44093, France.
Neurosci Lett. 2017 Feb 22;641:81-86. doi: 10.1016/j.neulet.2017.01.050. Epub 2017 Jan 23.
Lewy bodies and neurites, the pathological hallmarks found in the brain of Parkinson's disease (PD) patients, are primarily composed of aggregated and hyperphosphorylated alpha-synuclein. The observation that alpha-synuclein inclusions are also found in the gut of the vast majority of parkinsonian patients has led to an increasing number of studies aimed at developing diagnostic procedures based on the detection of pathological alpha-synuclein in gastrointestinal biopsies. The previous studies, which have all used immunohistochemistry for the detection of alpha-synuclein, have provided conflicting results. In the current survey, we used a different approach by analyzing the immunoreactivity pattern of alpha-synuclein separated by one- and two-dimensional electrophoresis, in colonic biopsies from PD subjects and healthy individuals. We did not observe any differences between controls and PD in the expression levels, phosphorylation or aggregation status of alpha-synuclein. Overall, our study suggests that the two biochemical methods tested here are not adequate for the prediction of PD using gastrointestinal biopsies. Further studies, using other biochemical approaches, are warranted to test whether there exists specific forms of pathological alpha-synuclein that distinguish PD from control subjects.
路易小体和神经突是帕金森病（PD）患者大脑中发现的病理特征，主要由聚集和过度磷酸化的α-突触核蛋白组成。绝大多数帕金森病患者的肠道中也发现了α-突触核蛋白包涵体，这一观察结果导致越来越多的研究旨在开发基于检测胃肠道活检中病理性α-突触核蛋白的诊断程序。之前的研究都使用免疫组织化学来检测α-突触核蛋白，但结果相互矛盾。在本次调查中，我们采用了不同的方法，通过分析PD患者和健康个体结肠活检中经一维和二维电泳分离的α-突触核蛋白的免疫反应模式。我们没有观察到对照组和PD患者在α-突触核蛋白的表达水平、磷酸化或聚集状态上有任何差异。总体而言，我们的研究表明，这里测试的两种生化方法不足以通过胃肠道活检来预测PD。有必要进行进一步的研究，采用其他生化方法来测试是否存在区分PD患者和对照受试者的病理性α-突触核蛋白的特定形式。
